Establishment of optimized in vitro disinfection protocol of Pistacia vera L. explants mediated a computational approach: multilayer perceptron-multi-objective genetic algorithm.

Background: Contamination-free culture is a prerequisite for the success of in vitro - based plant biotechnology. Aseptic initiation is an extremely strenuous stride, particularly in woody species. Meanwhile, over-sterilization is potentially detrimental to plant tissue.

Hydrotime analysis to explore the effect of HO-priming in the relationship between water potential (Ψ) and germination rate of Capsicum annuum L. seed under NaCl- and PEG-induced stress.

Hydrogen peroxide (H2O2) priming was proved to be effective to boost salt and drought stress tolerance in Capsicum annuum L. Hither, hydrotime (HT) approach, a population-based threshold model-germination dependence on water availability, was introduced to account for the effects of reduced water potential on HO-primed seeds in progress toward germination. Pre-optimized HO concentrations (1 and 10 mM for 24 h at 25 °C) were applied.

Somatic embryogenesis from seeds in a broad range of Vitis vinifera L. varieties: rescue of true-to-type virus-free plants.

Background: Somatic embryogenesis is the preferred method for cell to plant regeneration in Vitis vinifera L. However, low frequencies of plant embryo conversion are commonly found. In a previous work we obtained from cut-seeds of a grapevine infected with the Grapevine leafroll associated viruses 1 and 3 (GLRaV-1 and GLRaV-3), high rates of direct regeneration, embryo plant conversion and sanitation.