Elevation of ANXA1 associated with potential protective mechanism against ferroptosis and immune cell infiltration in age-related macular degeneration.

Background: Age-related macular degeneration (AMD), is a neurodegenerative ocular disease. This study investigated the role of ferroptosis-related genes and their interaction with immune cell infiltration in AMD.

Methods: We screened differential expression genes (DEGs) of AMD from data sets in Gene Expression Omnibus.

A Simple Repair Algorithm for Descemet's Membrane Detachment Performed at the Slit Lamp.

Our study aims to investigate a simple repair algorithm for Descemet's membrane detachment (DMD) following phacoemulsification with Pentacam and anterior segment optical coherence tomography (AS-OCT). Twelve patients with DMD were included in this retrospective study. All cases had persistent corneal edema after phacoemulsification and no improved response to conservative treatment.

A novel clinical dynamic stereopsis assessment based on autostereoscopic display system.

Background: Although it is recognized that dynamic stereopsis is vital in daily life, there is still room for improvement in assessment methods. A novel clinical dynamic stereopsis assessment method was created based on an autostereoscopic display system that did not require additional auxiliary glasses. This study evaluated the optical parameters and clinical performance of the autostereoscopic display system for clinical dynamic stereopsis assessment.

Galectin-3 Is a Crucial Immunological Disease Marker in Patients with Fungal Keratitis.

Fungal keratitis, one of the most common infectious eye diseases in China, often results in a poor prognosis due to a delayed diagnosis and the insufficiency of effective therapy. There is an urgent need to identify specific biomarkers for the disease. In this study, we screened out tear proteins in patients with fungal keratitis by microsphere-based immunoassay analysis.

Inhibition of Galectin-3 Impairs Antifungal Immune Response in Fungal Keratitis.

Galectin-3 is one of the galectin family members which are master regulators of immune homeostasis, especially in infectious diseases. However, its mechanism of immune regulation in fungal keratitis has not been thoroughly studied. Our study is aimed at clarifying the role of galectin-3 in the fungal keratitis mouse model in vivo, thereby providing a new biomarker of antifungal therapy.

Effects of Customized Progressive Addition Lenses vs. Single Vision Lenses on Myopia Progression in Children with Esophoria: A Randomized Clinical Trial.

Purpose: To evaluate the effect of customized progressive addition lenses (CPALs) versus single vision lenses (SVLs) on the progression of juvenile-onset myopia in children with near esophoria.

Methods: Ninety-three Chinese children, aged 7-14 years with spherical equivalent refraction (SER) ranging from -0.50 to -4.

Changes in Anterior and Posterior Corneal Elevation in Patients With Allergic Conjunctivitis.

To evaluate corneal elevation changes in patients with allergic conjunctivitis (AC) and to analyze their correlations with ocular allergy signs and corneal biomechanical alterations. Thirty patients (30 eyes) with AC and twenty normal subjects (20 eyes) were included in this prospective study. All participants underwent a complete ocular examination, including corneal tomography by Pentacam and corneal biomechanics evaluation by Corvis ST.

Effect of Dual Endothelin Receptor Antagonist on a Retinal Degeneration Animal Model by Regulating Choroidal Microvascular Morphology.

Objective: Clinical studies have found that increasing levels of plasma endothelin-1 (ET-1) might inhibit choroidal blood flow (BF) and promote choroidal vasoconstriction. This study was designed to investigate ET-1 levels and its effect on choroidal microvascular morphology in a retinitis pigmentosa (RP) animal model.

Methods: Mice with retinal degeneration (rd10) were intragastrically administered bosentan, a dual endothelin receptor antagonist.

Corneal Opacity Leading to Multiple Myeloma Diagnosis: A Case Report and Literature Review.

BACKGROUND A multiple myeloma patient presenting with corneal opacity and blurred vision as chief complaint is rare. CASE REPORT A 54-year-old woman with a 9-month history of blurred vision without other systemic symptoms consulted an ophthalmologist. The patient had bilateral diffuse corneal opacity at the corneal epithelium and anterior stroma under slit-lamp examination.

Valsalva Retinopathy in Twin-Pregnancy: A Case Report and Literature Review.

BACKGROUND Valsalva retinopathy is a rare but sight-threatening condition with sudden visual loss. Twin-pregnancy is known as a confirmed risk factor for Valsalva retinopathy. CASE REPORT A 21-year-old twin-pregnant Chinese woman with 31 weeks of gestation complained of a sudden decrease of visual acuity in her right eye for five hours.

Reconstruction of auto-tissue-engineered lamellar cornea by dynamic culture for transplantation: a rabbit model.

To construct an auto-tissue-engineered lamellar cornea (ATELC) for transplantation, based on acellular porcine corneal stroma and autologous corneal limbal explants, a dynamic culture process, which composed of a submersion culture, a perfusion culture and a dynamic air-liquid interface culture, was performed using appropriate parameters. The results showed that the ATELC-Dynamic possessed histological structure and DNA content that were similar to native lamellar cornea (NLC, p>0.05).

Determination of anterior segment changes with Pentacam after phacoemulsification in eyes with primary angle-closure glaucoma.

Background: To investigate changes of anterior segment morphology in primary angle-closure glaucoma after phacoemulsification using the Pentacam system.

Design: Prospective, interventional study, Zhongshan Hospital of Sun Yat-sen University.

Participants: Eighty-five eyes from 60 patients with primary angle-closure glaucoma undergoing phacoemulsification.

Long-term cultivation of human corneal endothelial cells by telomerase expression.

The objective of this study was to explore the potential role of human telomerase reverse transcriptase (TERT) in extending the proliferative lifespan of human corneal endothelial cells (HCECs) under long-term cultivation. A primary culture was initiated with a pure population of HCECs in DMEM/F12 media containing 10% fetal bovine serum and other various supplements. TERT gene was successfully transfected into normal HCECs.

Research on the stability of a rabbit dry eye model induced by topical application of the preservative benzalkonium chloride.

Background: Dry eye is a common disease worldwide, and animal models are critical for the study of it. At present, there is no research about the stability of the extant animal models, which may have negative implications for previous dry eye studies. In this study, we observed the stability of a rabbit dry eye model induced by the topical benzalkonium chloride (BAC) and determined the valid time of this model.

A modified symblepharon ring for sutureless amniotic membrane patch to treat acute ocular surface burns.

The objective of this study is to evaluate a sutureless technique by using a modified symblepharon ring to fix an amniotic membrane (AM) patch on the ocular surface to treat acute ocular burns. Seventy-five patients with acute ocular burns of total 75 eyes graded III to VI were enrolled in this study. They were randomly divided into two groups.

Tectonic lamellar keratoplasty with acellular corneal stroma in high-risk corneal transplantation.

Purpose: Tectonic lamellar keratoplasty (TLKP) is a primary surgical procedure to improve the condition of the recipient bed in high-risk corneal transplantation. It is usually performed for a secondary optical penetrating keratoplasty (PKP). The present study was undertaken to explore a new strategy for TLKP using acellular corneal stroma (ACS) prepared by decellularization.

Using acellular porcine limbal stroma for rabbit limbal stem cell microenvironment reconstruction.

To investigate the feasibility of using acellular porcine limbal stroma for limbal stem cell microenvironment reconstruction. Limbal reconstruction was performed in rabbit partial limbal defect models. Rabbits were randomly divided into four groups: acellular porcine limbal stroma, de-epithelized rabbit limbal autograft stroma, de-epithelized porcine limbal stroma and acellular porcine corneal stroma transplantation groups.

A novel procedure for treating canalicular obstruction by re-canaliculisation and bicanalicular intubation.

Aim: The aim of this study was to evaluate a new procedure for treating canalicular obstruction by re-canaliculisation and bicanalicular intubation (RC-BCI).

Methods: Thirty adult patients (32 eyes) with canalicular obstruction were treated with RC-BCI from September 2005 to December 2007 at Zhongshan Ophthalmic Centre (Guangzhou, China). Silicone tubes were left in place for 2-3 months and were removed when patients had relief by tearing.

Development and characterization of acellular porcine corneal matrix using sodium dodecylsulfate.

Purpose: The objective of this study was to produce a porcine corneal acellular matrix (ACM) and assess its possibility for biomedical applications.

Methods: Porcine corneas were treated with various concentrations of sodium dodecylsulfate for different lengths of time. Optimal conditions for processing the ACM were noted regarding removal of all cellular components and retention of the spatial arrangement of the corneal stroma.

The use of phospholipase A(2) to prepare acellular porcine corneal stroma as a tissue engineering scaffold.

This study was to develop a method using phospholipase A(2) (PLA(2)) to prepare acellular porcine corneal stroma (APCS) for tissue engineering. The APCS was prepared from native porcine cornea (NPC) that was treated with 200 U/ml PLA(2) and 0.5% sodium deoxycholate (SD).

A rabbit dry eye model induced by topical medication of a preservative benzalkonium chloride.

Purpose: To establish a rabbit dry eye model with topical medication of the ocular preparation preservative benzalkonium chloride (BAC).

Methods: Sixteen white rabbits were used. One eye of each rabbit was chosen randomly for topical administration of 0.

Sutureless fixation of amniotic membrane patch as a therapeutic contact lens by using a polymethyl methacrylate ring and fibrin sealant in a rabbit model.

Purpose: To introduce and evaluate a sutureless technique by using a polymethyl methacrylate (PMMA) ring and fibrin sealant to fix an amniotic membrane (AM) patch on the ocular surface as a therapeutic contact lens in a rabbit model.

Methods: PMMA rings were fabricated by duplicating an impression of a rabbit conjunctival fornix. The central cornea of the left eye in 16 rabbits was deepithelialized (diameter = 10 mm).