A pan-genome perspective on the evolutionary dynamics of polyphyly, virulence, and antibiotic resistance in serovar Mbandaka highlights emerging threats to public health and food safety posed by cloud gene families.

serovar Mbandaka, a prevalent foodborne pathogen, poses a threat to public health but remains poorly understood. We have determined the phylogenomic tree, genetic diversity, virulence, and antimicrobial resistance (AMR) profiles on a large genomic scale to elucidate the evolutionary dynamics within the Mbandaka pan-genome. The polyphyletic nature of this serovar is characterized by two distinct phylogenetic groups and inter-serovar recombination boundaries, that potentially arising from recombination events at the H2-antigen loci.

Absolute quantification of viable in seawater samples using multiplex droplet digital PCR combined with propidium monoazide.

Introduction: Toxigenic serogroup O1 and O139 are the pathogens responsible for the global cholera epidemic. can settle in the water and spread the fecal-oral route. Rapid and accurate monitoring of live in environmental water has become an important strategy to prevent and control cholera transmission.

p43 induces IP-10 expression through the JAK-STAT signaling pathway in HMEC-1 cells.

p43 is a cofactor of aminoacyl-tRNA synthetase in mammals that effectively inhibits angiogenesis. However, the role of p43 in angiogenesis remains unclear. In the present study, we examined the effects of p43 on angiogenesis using human microvascular endothelial cells-1 (HMEC-1) cells as a model.

Construction of 2,4,6-Trinitrotoluene Biosensors with Novel Sensing Elements from Escherichia coli K-12 MG1655.

Detection of 2,4,6-trinitrotoluene (TNT) has been extensively studied since it is a common explosive filling for landmines, posing significant threats to the environment and human safety. The rapid advances in synthetic biology give new hope to detect such toxic and hazardous compounds in a more sensitive and safe way. Biosensor construction anticipates finding sensing elements able to detect TNT.