Effect of Collagen Nanofibers and Silanization on the Interaction of HaCaT Keratinocytes and 3T3 Fibroblasts with Alumina Nanopores.

During wound healing, a complex cascade of cellular and molecular events occurs, which is governed by topographical and biochemical cues. Therefore, optimal tissue repair requires scaffold materials with versatile structural and biochemical features. Nanoporous anodic aluminum oxide (AAO) membranes exhibit good biocompatibility along with customizable nanotopography and antimicrobial properties, which has brought them into the focus of wound treatment.

Self-assembled fibrinogen nanofibers support fibroblast adhesion and prevent E. coli infiltration.

Fibrinogen nanofibers hold great potential for wound healing applications since they mimic the native blood clot architecture and offer important binding sites to support fibroblast adhesion and migration. Recently, we introduced a new method of salt-induced self-assembly to prepare nanofibrous fibrinogen scaffolds. Here, we present our results on the mechanical properties of these scaffolds and their interaction with 3T3 fibroblasts and E.

Spatial patterning of nanofibrous collagen scaffolds modulates fibroblast morphology.

Current knowledge about cell-biomaterial interactions is often based on two-dimensional (2D) cell culture systems like protein-coated glass slides. However, such smooth surfaces cannot mimic the nanofibrous environment of the native extracellular matrix (ECM). It is therefore a major challenge to transfer the results from 2D surfaces to 3D protein scaffolds with biomimetic nanofiber architecture.

Wet-spinning of magneto-responsive helical chitosan microfibers.

Helical structures can be found in nature at various length scales ranging from the molecular level to the macroscale. Due to their ability to store mechanical energy and to optimize the accessible surface area, helical shapes contribute particularly to motion-driven processes and structural reinforcement. Due to these special features, helical fibers have become highly attractive for biotechnological and tissue engineering applications.

Controlling the Multiscale Structure of Nanofibrous Fibrinogen Scaffolds for Wound Healing.

As a key player in blood coagulation and tissue repair, fibrinogen has gained increasing attention to develop nanofibrous biomaterial scaffolds for wound healing. Current techniques to prepare protein nanofibers, like electrospinning or extrusion, are known to induce lasting changes in the protein conformation. Often, such secondary changes are associated with amyloid transitions, which can evoke unwanted disease mechanisms.