Purification, structure characterization and antioxidant activity of polysaccharides from Saposhnikovia divaricata.

Polysaccharide from traditional Chinese herb, Saposhnikovia divaricata (Turcz.) Schischk. (SD) was extracted, fractionated and characterized in this work.

Protective effects of Rabdosia japonica var. glaucocalyx extract on lipopolysaccharide-induced acute lung injury in mice.

The present study was designed to evaluate the protective effects of ethanol extracts of Rabdosia japonica var. glaucocalyx (Maxim.) Hara (RJ) on lipopolysaccharide (LPS)-induced acute lung injury (ALI) in mice and the possible underlying mechanisms of action.

Rabdosia japonica var. glaucocalyx Flavonoids Fraction Attenuates Lipopolysaccharide-Induced Acute Lung Injury in Mice.

Rabdosia japonica var. glaucocalyx (Maxim.) Hara, belonging to the Labiatae family, is widely used as an anti-inflammatory and antitumor drug for the treatment of different inflammations and cancers.

[Chemical constituents of Rabdosia japonica var. glaucocalyx and their anti-complementary activity].

To study the chemical constituents of Rabdosia japonica var. glaucocalyx and their anti-complementary activity on the basis of preliminary studies. Target isolation guided by anti-complementary activity test, compounds in the chloroform and n-butanol fractions were isolated and purified by silica gel and Sephadex LH-20 column chromatographies, and preparative HPLC.

Three new acyclic diterpenoids from Eupatorium lindleyanum DC.

Three new acyclic diterpenoids were isolated from the whole plant of Eupatorium lindleyanum DC. The structures of the compounds were elucidated by means of (1)H and (13)C NMR spectroscopic analyses, including 2D NMR experiments.

Proteomic analysis of lung tissue of rats exposed to cigarette smoke and radon.

This study examined the protein expression in lung tissues of rats exposed to radon and cigarette smoke using a proteomic approach. Male Wistar rats were exposed daily to radon at a concentration of 100,000 Bq/m(3) for 16 h, and then exposed to 20% cigarette smoke for 1 h for a period of 75 d, with the radon cumulative dose reaching 200 WLM (working level months). Proteins from rat lung tissue were separated by two-dimensional gel electrophoresis (2-DE), stained with Coomassie blue, and analyzed with ImageMaster two-dimensional (2D) platinum software.

Radon-induced proteomic profile of lung tissue in rats.

The aim of this study was to investigate the differential expression of proteins in lung of rats following long-term exposure to radon. The total proteins of lung tissue from Wistar rats exposed to radon for cumulative doses up to 100, 200, or 400 WLM (working level months) were isolated by two-dimensional electrophoresis (2-DE) and analyzed with ImageMaster 2D Platinum software. Comparison of the 2-DE images between the control and radon-exposed groups resulted in 14 upregulated and 9 downregulated protein spots, of which 15 were identified by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) or matrix-assisted laser desorption/ionization time-of-flight/time-of-flight mass spectrometry (MALDI-TOF/TOF-MS).

[Experimental study on treatment of allergic asthma guinea pigs with different fractions of Modified Zhisousan].

Objective: To study the effects of different fractions of Modified Zhisousan (MZ, MZC, MZS) on the contents of nitric oxide (NO), endothelin-1 (ET-1) and eosinophilia (EOS) in the allergic asthma guinea pigs and observe the pathology changes of lung tissue.

Methods: The number of EOS in the blood and bronchialveolar lavage fluid (BALF) was counted by Wright staining. The contents of ET-1 and No in serum and BALF were analyzed by RIA and nitric acid reductase method.