Visual signal transduction for environmental stewardship: A novel biosensing approach to identify and quantify chlorpyrifos-related residues in aquatic environments.

The pervasive presence of organophosphate pesticides (OPs), such as chlorpyrifos (CPF), in aquatic ecosystems underscores the urgent need for sensitive and reliable detection methods to safeguard environmental and public health. This study addressed the critical need for a novel biosensor capable of detecting CPF and its toxic metabolite, 3,5,6-trichloro-2-pyridinol (TCP), with high sensitivity and selectivity, suitable for field applications in environmental monitoring. The study engineered a whole-cell biosensor based on E.

Detection of Cadmium in Human Biospecimens by a Cadmium-Selective Whole-Cell Biosensor Based on Deoxyviolacein.

Cadmium poses a severe health risk, impacting various bodily systems. Monitoring human exposure is vital. Urine and blood cadmium serve as critical biomarkers.

High-throughput screening of human mercury exposure based on a low-cost naked eye-recognized biosensing platform.

Whole-cell biosensors could be helpful for in situ disease diagnosis. However, their use in analyzing biological samples has been hindered by unstable responses, low signal enhancement, and growth inhibition in complex media. Here, we offered a solution by building a visual whole-cell biosensor for urinary mercury determination.

Pb(II)-inducible proviolacein biosynthesis enables a dual-color biosensor toward environmental lead.

With the rapid development of synthetic biology, various whole-cell biosensors have been designed as valuable biological devices for the selective and sensitive detection of toxic heavy metals in environmental water. However, most proposed biosensors are based on fluorescent and bioluminescent signals invisible to the naked eye. The development of visible pigment-based biosensors can address this issue.

Metabolic engineering of the carotenoid biosynthetic pathway toward a specific and sensitive inorganic mercury biosensor.

The toxicity of mercury (Hg) mainly depends on its form. Whole-cell biosensors respond selectively to toxic Hg(ii), efficiently transformed by environmental microbes into methylmercury, a highly toxic form that builds up in aquatic animals. Metabolically engineered () have successfully produced rainbow colorants.

Metabolic engineering of the violacein biosynthetic pathway toward a low-cost, minimal-equipment lead biosensor.

Metabolic engineered bacteria have been successfully employed to produce various natural colorants, which are expected to be used as the visually recognizable signals to develop mini-equipment biological devices for monitoring toxic heavy metals. The violacein biosynthetic pathway has been reconstructed in Escherichia coli (E. coli).

Indigoidine biosynthesis triggered by the heavy metal-responsive transcription regulator: a visual whole-cell biosensor.

During the last few decades, whole-cell biosensors have attracted increasing attention for their enormous potential in monitoring bioavailable heavy metal contaminations in the ecosystem. Visual and measurable output signals by employing natural pigments have been demonstrated to offer another potential choice to indicate the existence of bioavailable heavy metals in recent years. The biosynthesis of the blue pigment indigoidine has been achieved in E.

Versatile artificial mer operons in Escherichia coli towards whole cell biosensing and adsorption of mercury.

Mercury exists naturally and mainly as a man-made pollutant in the environment, where it exerts adverse effects on local ecosystems and living organisms. It is important to develop an appropriate synthetic biological device that recognizes, detects and removes the bioavailable fraction of environmental mercury. Both single-signal and double-signal output mercury biosensors were assembled using a natural mer operon as a template.

Development of Cadmium Multiple-Signal Biosensing and Bioadsorption Systems Based on Artificial Operons.

The development of genetic engineering, especially synthetic biology, greatly contributes to the development of novel metal biosensors. The operon encoding cadmium resistance was previously characterized from . In this study, single-, dual-, and triple-signal output Cd(II) biosensors were successfully developed using artificial translationally coupled operons.

Genetic control of violacein biosynthesis to enable a pigment-based whole-cell lead biosensor.

Environmental risks continue to grow due to heavy metal contamination caused by anthropogenic activities. Accumulation of harmful quantities of lead poses a threat to aquatic organisms, plants, and human beings. Whole-cell biosensors, which can proliferate independently, can detect the bioavailable fraction to assess the effect of target heavy metal on the environmental ecosystem.

Vibrio profundi sp. nov., isolated from a deep-sea seamount.

A Gram-stain negative, rod-shaped, facultative anaerobic, motile bacterial strain, designated TP187, was isolated from a seamount near the Yap Trench in the tropical western Pacific. Phylogenetic analysis based on the 16S rRNA gene sequence showed that strain TP187 is related to members of the genus Vibrio and has high 16S rRNA gene sequence similarity with the type strains of Vibrio chagasii (97.3%) and Vibrio gallaecicus (97.

[Heavy metals in the surface sediment of the dumping ground outside Jiaozhou Bay and their potential ecological risk].

Based on the monitoring data of heavy metals (Cr, Hg, Cd, Pb, Zn, Cu) in the surface sediment of the dumping ground outside Jiaozhou Bay from 2003 to 2008, the distribution patterns, factors controlling the distribution, and the potential ecological risks of heavy metals were studied with the data in 2007-08, and the fluctuation trends of heavy metals in the surface sediment over the 6 years were also discussed. The average concentrations of heavy metals Cr, Hg, Cd, Pb, Zn, Cu in the surface sediment were 29.47, 0.

[Marine inorganic carbon system responses to macro-DIN supply coupled with Ulva pertusa in simulated experiments].

Effects of macronutrient (NO3(-) -N and NH4(+) -N) on inorganic carbon system of water with Ulva pertusa existed were studied in laboratory simulation experiments. The results demonstrated that nutrient enrichment induced DIC, HCO3- and p(CO2) decreased while pH and CO3(2-) increased. The seawater changed from carbon source to carbon sink.

[Effects of excessive inorganic nitrogen on inorganic carbon system in seawater simulated experiments].

The influence of excessive inorganic nitrogen (NO3-, NH4+, NO2-) on the pH, inorganic carbon system and air-sea CO2 flux was studied in laboratory seawater simulation experiments. The result indicates that the excessive inorganic nitrogen significantly has an important influence on the inorganic carbon system in all conditions. At the NO3- addition groups, HCO3- and p (CO2) increase while pH and CO3(2-) decrease when the concentration of NO3- < or = 37.

[Variation characteristics and controlling factors of heavy metals in the South Yellow Sea surface seawaters].

Based on the 8 cruises data of surface seawater heavy metals and other related environmental factors from 1997 to 2004 of the South Yellow Sea (SYS), distribution patterns, mechanisms controlling the distributions and pollution levels of heavy metals (As, Cd, Cu, Hg, Pb, Zn) were studied with the data of 2003-10, and 8-year-fluctuation trends of heavy metals were also discussed. The average concentrations of heavy metals in surface seawater were 2.33, 0.