[Molecular evolution of GAGE gene family].

The gene family GAGE is characterized to be expressed in testis and a portion of tumors, which is considered to be a candidate for diagnostic marker and immunotherapy target. The present study revealed that GAGE gene is unique to primate lineage. At least 15 duplicates with low divergence were found to be clustered in human X chromosome, while chimpanzee and macaque has 3 and 4 in a similar manner.

Protein and mRNA characterization in high and low metastasis adenoid cystic carcinoma cell lines.

Metastasis and invasion, the important characteristics of malignant tumors, are closely associated with a series of changes in the expression of genes and proteins. In this study, we compare mRNA and protein expression in high and low metastasis adenoid cystic carcinoma cell lines by mRNA suppression subtractive hybridization and two-dimensional electrophoresis combined with peptide mass fingerprint analysis. 34 differentially expressed genes were obtained using suppression subtractive hybridization experiments including 6 highly expressed gene sequences in the high metastasis cell line, and 28 in the low metastasis cell line.

[Gene expression differences between high and low metastatic cells of adenoid cystic carcinoma].

Objective: To investigate the complex differences between high metastatic and low metastatic cells of the Adenoid cystic Carcinoma.

Methods: Gene expression patterns were examined in high metastatic cell ACC-M strain and low metastatic ACC-2 strain with the method SSH (suppression subtractive hybridization).

Results: although extensive similarity was noted between the expression profiles, twelve genes were highly expressed of, in low metastatic cell ACC-2 tester, compared with driver, high metastatic cell ACC-M.

Expression of the negative co-stimulatory ligand sCD152 in the yeast, Pichia pastoris, and its regulation of antigen specific immune responses.

CD152, a ligand expressed on the surface of the activated T cells that inhibits co-stimulatory signals, is associated with negative regulation of T-cell activation in the antigen-presenting process. In order to interfere with immune signal transmission, obtain functional proteins with specific immunosuppressive effects, and regulate the immune response, we cloned the full-length extracellular domain of CD152 into the expression vector pPICTLA and transformed Pichia pastoris GS115 cells by electroporation. Yeast colonies expressing and secreting large quantities of the extracellular soluble fragment of CD152 (sCD152) were isolated, and the protein was purified and used in assays designed to investigate the ability of sCD152 to regulate the immune system.

Study of the difference of high and low metastasis cell line's gene expression map and metastasis-related genes of adenoid cystic carcinoma.

We searched for metastasis-related genes in adenoid cystic carcinoma by suppression subtractive hybridization analysis of high and low metastasis cell lines. Twelve genes (ten previously identified and two novel sequences) were identified as being expressed at lower levels in high metastasis cell line Acc-M when compared to low metastasis cell line Acc-2. The known sequences corresponded to the genes for cysteine-rich angiogenesis induction factor (cyr61), chromosome 7 RP11-52501 clone, G-protein, WAS familial ferritin I heavy chain, jumping translocation breakpoint, eukaryotic translation elongation, folate receptor and three ribosomal proteins.