[Expression and purification of avian influenza virus H5N1 NP protein, and screening interaction proteins of host cells in vitro].

Objective: To express and purify H5N1 influenza virus (A/Anhui/1/2005) NP in prokaryotic system and to explore the NP-interacting proteins of human bronchial epithelial cells BEAS-2B in vitro.

Methods: The full length H5N1 NP gene fragment was amplified by PCR, inserted into prokaryotic expression vector (pET30a) to generate NP expression plasmid pET30a-NP. After transforming pET30a-NP into E.

[Study on the immunogenicity of adeno-vector vaccine against H5N1 influenza A virus].

Objective: To construct adenovirus vector vaccine against H5N1 influenza virus and study on the immunogenicity.

Methods: In this study, we amplified hemagglutinin (HA) gene sequence of H5N1 influenza virus (A/Anhui/1/2005), then constructed an adenovirus vector vaccine (Adv-HA), followed by tests in BALB/c mice for the immunogenicity with the vaccine and immunization strategies.

Results: The recombinate Adv-HA vaccine could effectively induce both humoral and cellular immunity against human H5N1 influenza virus.

Nonopsonic phagocytosis of Lactobacilli by mice Peyer's patches' macrophages.

The ingestion of lactobacilli is of great importance for the probiotic effect of host gut Peyer's patches (PPs) macrophages. The present study is in time focus on the investigation of the factors determining the ingestion of lactobacilli by PPs macrophages. Physicochemical properties of cell surface and adhesive property of nine Lactobacillus strains were examined in the present work.

Is delayed gastric emptying so terrible after pylorus-preserving pancreaticoduodenectomy? Prevention and management.

Aim: To explore some operative techniques to prevent the occurrence of delayed gastric emptying (DGE) after pylorus-preserving pancreaticoduodenectomy (PPPD).

Methods: One hundred and eighty-six patients in a single medical center who accepted PPPD were retrospectively studied. The incidence of DGE was investigated and the influence of some operative techniques on the prevention of DGE was analyzed.