Effect of morphine and a low dose of ketamine on the T cells of patients with refractory cancer pain .

The combination of morphine and ketamine is considered safe and efficacious in many patients. However, a considerable number of immunomodulatory effects have been reported to be produced by both morphine and ketamine. The aim of the present study was to assess the direct effect of morphine and a low dose of ketamine on the T cells of patients with refractory cancer pain .

Morphine and ketamine inhibit immune function of gastric cancer patients by increasing percentage of CD4(+)CD25(+)Foxp3(+) regulatory T cells in vitro.

Background: There is conflicting evidence regarding effects of anesthetic and analgesic drugs on immune function of cancer patients. This study was designed to observe changes of T cell subpopulations in the gastric cancer (GC) patients and to assess effects of morphine and ketamine on the CD4(+) T cells, CD8(+) T cells, and regulatory T cells (Tregs) populations obtained from the GC patients in vitro.

Methods: The peripheral blood samples from 20 GC patients and 20 healthy volunteers were obtained.

Effects of different concentrations of oxygen-ozone on rats' astrocytes in vitro.

Although the widespread use of the oxygen-ozone in pain management, there is currently no consensus on its mechanisms of action and nearly no report for its action on nervous cells. Accordingly, the present study was designed to assess the effects of oxygen-ozone on astrocytes. Astrocytes were cultured in vitro through methods of trypsinization, different-speed cultivation and passaging to purify, then seeded into 24 well plates and divided to one of four groups (n=7) to receive the following treatments: respectively added 400 microl complete medium (CM) after effects of 20 microg/ml oxygen-ozone (Group O-20), 40 microg/ml oxygen-ozone (Group O-40), 60 microg/ml oxygen-ozone (Group O-60); without intervention (Group C).