[The cytogenetic and molecular genetic study of 81 multiple myeloma patients].

Objective: To explore the cytogenetic characteristics of multiple myeloma (MM) patients, to evaluate the effect of a long-term culture stimulated by cytokines on cytogenetic study of MM, and to investigate the clinical detection value of RB1 and P53 deletion in interphase plasma cells by using fluorescence in situ hybridization (FISH).

Methods: Karyotype analysis was performed in 81 MM patients by using the short-term culture of bone marrow cell and G-banding technique. Among the 81 MM patients, 28 patients used two culture methods: one was the short-term culture and the other was to culture cells for 6 days with recombinant granulocyte-macrophage colony-stimulating factor (GM-CSF) (40 µg/L) and IL-6 (10 µg/L).

[The cytogenetic characteristics of 178 acute myeloid leukemia patients].

Objective: To explore the cytogenetic characteristics of acute myeloid leukemia (AML) patients.

Methods: The karyotype analysis was performed in 178 AML using the short-term culture of bone marrow cell and G-banding technique.

Results: Among the 178 patients, 171 had enough metaphases for analysis and 128 (74.

[Telomere length and telomerase expression activity in mononuclear cells of patients with chronic lymphocytic leukemia].

This study was aimed to detect the telomere length and the telomerase expression activity in patients with chronic lymphocytic leukemia (CLL), and investigate their relation to prognosis of CLL. The telomere length and the telomerase expression activity of peripheral blood and / or bone marrow mononuclear cells were examined by Tel-FISH, a semi-quantitative method and by TRAP-ELISA respectively; the expressions of ZAP70 and CD38 were detected by flow cytometry. The results showed that comparing the telomere length in different stages, there was a tendency that the telomere became prolonged when the stage raised up.

[Changes of pathogens and susceptibility to antibiotics in hematology ward from years 2001 to 2005].

The purpose of this study was to determine the changes of pathogens in hematological ward and susceptibility of patients received chemotherapy to antibiotics. The pathogens were taken from blood, urine and sputum of patients who accepted chemotherapy from years 2001 to 2005, then were isolated and identified. The susceptibility test was performed by disk diffusion method.

[A clinical study of 103 idiopathic thrombocytopenic purpura].

Objective: To investigate the prevalence by age, response to different therapies and outcome in newly diagnosed idiopathic thrombocytopenic purpura (ITP).

Methods: ITP patients who were hospitalized from July 1992 to December 2006 and followed up with telephone were retrospectively analyzed.

Results: 103 patients with ITP were investigated.

[Construction of fusion gene between IgGHV and IL-2 as IgHV nucleic acid vaccine against lymphoma].

The purpose of this study was to construct the IgHV and IL-2 coexpressed vector. The IgHV gene fragments were obtained from the peripheral blood of patients with lymphoma, and were cloned into eukaryotic expression vector. Meanwhile, the gene fragments of IgHV linked with gene of IL-2 were inserted into pcDNA3.

[Long-term expansion of T cell progenitors by JAK3 gene transduced primitive hematopoietic cells in vitro].

Objective: To explore whether activation of the JAK3-signaling pathway can stimulate long-term expansion of the earliest T cell progenitors from transduced primitive hematopoietic cells and evaluate their potential ability of committed differentiation.

Methods: A retrovirus vector (RV) containing JAK3 gene, two binding sites for chemical inducers of dimerization (AP20187), and green fluorescence protein (GFP), MGI-F(2)JAK3, was constructed. The RV vector MGI-F(2)JAK3 was then transduced into murine bone marrow hematopoietic cells.

[Promotion of in vitro long term expansion of primitive multipotential hematopoietic cells by transduced JAK2 gene].

Objective: To explore whether activation of the JAK2-signaling pathway can stimulate long-term expansion and regulation of hematopoietic stem cells/multipotential hematopoietic progenitor cells (HSC/MHPC), and evaluate their potential ability of committed differentiation.

Methods: A retrovirus vector (RV) which contains JAK2 gene and two binding sites for a chemical inducers of dimerization (AP20187) was constructed. JAK2 can be dimerized by adding AP20187.

[Long term regulated expansion and committed differentiation of JAK2 gene transfected hematopoietic stem/progenitor cells in vitro].

Objective: To explore the feasibility of regulated expansion and committed differentiation potential of JAK2 gene modified hematopoietic stem/progenitor cells in vitro.

Methods: A murine stem cell virus (MSCV) based retroviral vector MGI-F2Jak2, which encodes a green fluorescent protein (GFP) and a fusion protein containing two copies of modified FK506 binding protein (F36v) linked tyrosine kinase JAK2 was cloned. F36v served as a high-affinity binding site for dimerizer AP20187.