[Effects of Histone Methyltransferase Inhibitors on the Survival, Apoptosis and Cell Cycle of Raji Cells].

Objective: To determine the effects of three histone methylase inhibitors UNC1999, DZNep and GSK343 on the survival, apoptosis and cell cycle of non-hodgkin's lymphoma Raji cells.

Methods: PCR amplified 16 and 18 exons of enhancer of zeste homolog 2 ( 2) gene were detected. The expression of EZH2 in normal adult lymphocytes and Raji cells was detected by Western blot.

Arg124Cys mutation of the TGFBI gene in a Chinese pedigree of Reis-Bücklers corneal dystrophy.

Aim: To analyze mutations in transforming growth factor beta-induced (TGFBI) gene in a Chinese pedigree with Reis-Bücklers corneal dystrophy (RBCD, also known as GCD3).

Methods: In a five-generation Chinese family, eight members were identified with RBCD and the rest were unaffected. All members of the family underwent complete ophthalmologic examinations.

Beclin 1-mediated macroautophagy involves regulation of caspase-9 expression in cervical cancer HeLa cells.

Objective: To investigate the role of Beclin 1 in HeLa cells and to obtain further insight into the relationship between autophagy and apoptosis.

Methods: Beclin 1 silencing was achieved using RNA interference. The expression of gene was measured using quantitative real time RT-PCR and Western blotting.

[Expression of glucocorticoid receptor alpha and beta mRNA in orbital tissues in thyroid associated ophthalmopathy].

Objective: To evaluate the expression of glucocorticoid receptor alpha (GRalpha) and beta (GRbeta) messenger RNA (mRNA) in orbital tissues from thyroid associated ophthalmopathy (TAO).

Methods: Samples of extraocular muscle and orbital fat were obtained from 17 patients with TAO and 10 healthy individuals. Total RNA was extracted and reversely transcripted into cDNA.

[Construction of shRNA expression vectors for autophagy gene Beclin 1 and effect of the vectors on transfected HeLa cells in vitro].

Objective: Autophagy gene Beclin 1 plays an important role in several types of human cancer. In this study, RNA interference (RNAi) technique was employed to determine the effect of inhibiting Beclin 1 on the growth of tumor cells.

Methods: According to the encoding sequence of mRNA of Beclin 1, the target site for the RNAi technique was designed and the vector for shRNA (short hairpin RNA) expression in tumor cells was constructed.

[Effect of adenovirus-mediated mda-7/IL-24 gene infection on apoptosis of drug-resistant human ovarian cancer cell lines OVCAR-3 and OVCAR-8/TR].

Background & Objective: Melanoma differentiation associated gene-7 (mda-7/IL-24) has double functions: specifically induces tumor cell apoptosis and modulates immune responses. Therefore, it is a strong candidate for human cancer gene therapy. This study was to evaluate the effect of adenovirus-mediated mda-7/IL-24 infection on the apoptosis of drug-resistant ovarian cancer cell lines OVCAR-3 and OVCAR-8/TR.

[Correlation of autophagy gene Beclin1 to tumorigenesis and development of epithelial ovarian cancer].

Background & Objective: Some studies have showed that autophagy suppression may result in malignant transformation, and the inactivation of autophagy gene Beclin1 induces malignancy. This study was to investigate the role of Beclin1 in the tumorigenesis and development of epithelial ovarian carcinoma, and to explore the effect of Beclin1 overexpression on the growth of ovarian carcinoma cell line SKOV3 in vitro.

Methods: The expression of Beclin1 in 25 specimens of normal ovarian tissue, 25 specimens of benign ovarian neoplasia, 19 specimens of borderline ovarian tissue, and 69 specimens of epithelial ovarian carcinoma was detected by immunohistochemistry.

[Expression of PPAR-gamma in human hilar bile duct carcinoma cell line QBC939 and the effects of PPAR-Y activated by its ligand on the growth of QBC939].

Objective: To explore the expression of Peroxisome Proliferator-activated Receptor-gamma (PPAR-gamma) in QBC939 and the effects of PPAR-gamma activated by its ligand pioglitazone on the growth of human bile duct carcinoma cell line.

Methods: QBC939 cells were cultured and treated with different concentration of pioglitazone; the expression of PPAR-gamma mRNA was detected by RT-PCR; the effects of PPAR-gamma activated by its ligand on cell proliferation were examined by cell count under light microscope; the influences of activated PPAR-gamma on cell cycle were examined by flow cytometry, and the apoptosis of cancer cells induced by PPAR-gamma ligand pioglitazone was detected by flow cytometry and TUNEL methods.

Results: PPAR-gamma was expressed in human hilar bile duct carcinoma cell line QBC939.

[Gene mutation analysis of coagulation factor VIII from a female patient with hemophilia A].

Hemophilia A affects male, whereas females are carriers and generally spared from this disease. However, we here reported a 65-year-old female with Hemophilia A while screening the gene mutation of coagulation factor VIII. The female went to hospital because of tripping to lead her right chest to be injured with subcutaneous hematoma.

Hypoxia-inducible transcription factor-1alpha promotes hypoxia-induced A549 apoptosis via a mechanism that involves the glycolysis pathway.

Background: Hypoxia-inducible transcription factor-1alpha (HIF-1alpha), which plays an important role in controlling the hypoxia-induced glycolysis pathway, is a "master" gene in the tissue hypoxia response during tumor development. However, its role in the apoptosis of non-small cell lung cancer remains unknown. Here, we have studied the effects of HIF-1alpha on apoptosis by modulating HIF-1alpha gene expression in A549 cells through both siRNA knock-down and over-expression.