Cationic copolymers that enhance wild-type-specific suppression in BNA-clamp PCR and preferentially increase the of fully matched complementary DNA and BNA strands.

Mutation detection is of major interest in molecular diagnostics, especially in the field of oncology. However, detection can be challenging as mutant alleles often coexist with excess copies of wild-type alleles. Bridged nucleic acid (BNA)-clamp PCR circumvents this challenge by preferentially suppressing the amplification of wild-type alleles and enriching rare mutant alleles.