Publications by authors named "Naho Okamoto"

(1) Background: Vitamin B deficiency in results in severe oxidative stress and induces morphological abnormality in mutants due to disordered cuticle collagen biosynthesis. We clarified the underlying mechanism leading to such mutant worms due to vitamin B deficiency. (2) Results: The deficient worms exhibited decreased collagen levels of up to approximately 59% compared with the control.

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In this study, we determined the vitamin B content of commercially-available edible insect products using a bioassay based on Lactobacillus delbrueckii ATCC 7830. Although the vitamin content of giant water bug, bee larva, grasshopper, and weaver ant products was low, we found that diving beetle and cricket products contained relatively high amounts of vitamin B (approximately 89.5 and 65.

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Ascorbate (AsA) is required as a cofactor and is widely distributed in plants and animals. Recently, it has been suggested that the nematode also synthesizes AsA. However, its biosynthetic pathway is still unknown.

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Article Synopsis
  • Adenyl cobamide, or pseudovitamin B, is produced by intestinal bacteria or found in certain cyanobacteria, but its impact on mammalian enzymes has not been extensively researched.
  • This study tested how pseudovitamin B influences the enzymes methionine synthase and methylmalonyl-CoA mutase in mammalian cells.
  • Results showed that while the hydroxo form can work as a coenzyme for methionine synthase, pseudovitamin B does not enhance its translation and doesn’t affect methylmalonyl-CoA mutase; it may also hinder the absorption of vitamin B by interfering with transcobalamin II.
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Food additives, such as hypochlorous acid water, sodium metabisulfite, and sodium sulfite, strongly affect the chemical and biological properties of vitamin B (cyanocobalamin) in aqueous solution. When cyanocobalamin (10 μmol/L) was treated with these compounds, hypochlorous acid water (an effective chlorine concentration of 30 ppm) rapidly reacted with cyanocobalamin. The maximum absorptions at 361 and 550 nm completely disappeared by 1 h, and vitamin B activity was lost.

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This study investigated the effect of various concentrations of pteroyl-mono-γ-glutamate (PteGlu) and pteroyl-di-γ-glutamate (PteGlu) on the growth of Lactobacillus rhamnosus strains ATCC 7469 (wild-type strain) and ATCC 27773 (chloramphenicol-resistant strain) used for folate microbiological assays. At concentrations of 0.025-0.

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Vitamin B deficiency leads to various symptoms such as neuropathy, growth retardation, and infertility. Vitamin B functions as a coenzyme for two enzymes involved in amino acid metabolisms. However, there is limited information available on whether amino acid disorders caused by vitamin B deficiency induce such symptoms.

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