Publications by authors named "Nahla Hussein"
Article Synopsis
- - The study focuses on Hyalomma dromedarii ticks, which are significant for transmitting pathogens to large animals in Egypt, especially as camels and cattle are imported from neighboring countries.
- - Researchers analyzed the transcriptome of these ticks by examining mixed mRNAs from various life stages (eggs, larvae, nymphs, and both fed and unfed adults) to understand the biochemical interactions that take place during tick feeding.
- - The results included a comparison of identified sequences with databases to identify potential protein functions and properties, setting the stage for further understanding of tick physiology and its implications for disease transmission in livestock.
Article Synopsis
- Monoclonal IgY antibodies are gaining popularity due to their high specificity and scalability, making them a strong alternative to traditional polyclonal IgY antibodies.
- The text outlines the production and purification methods of monoclonal IgY antibodies, emphasizing their advantages, such as superior reliability and cost-effectiveness, especially in low- and middle-income countries.
- It concludes by noting the significant benefits of monoclonal IgY antibodies for diagnostics, therapeutics, and research, while also addressing future challenges in the field.
Ticks have harmful impacts on both human and animal health and cause considerable economic losses. Leucine aminopeptidase enzymes (LAP) play important roles during tick infestation to liberate vital amino acids necessary for growth. The aim of the current study is to identify, express and characterize the LAP from the hard tick Hyalomma dromedarii and elucidate its biochemical characteristics.
View Article and Find Full Text PDFEscherichia coli cell envelope is crucial for stress sensing and signal transduction, mediated by numerous protein-protein interactions to enable adaptation and survival. Interfering with these interactions might affect envelope integrity leading to bacterial death. The outer membrane lipoprotein (RcsF) is the stress sensor of the regulator of capsule synthesis (Rcs) phosphorelay that senses envelope threats.
View Article and Find Full Text PDFSeveral diagnostic measures have been employed to precisely detect the SARS-CoV-2 viral infection using viral antigens, nucleic acids, and other serological approaches. The sensitivity and specificity of the serological tests remain a challenging need. Here, we describe the detection of human anti-SARS-CoV-2 IgG and IgM antibodies qualitatively through two optimized in-house ELISA and lateral flow immunoassay.
View Article and Find Full Text PDFArticle Synopsis
- The study focuses on how microorganisms, particularly the model organism K-12 MG1655, adapt to stressful conditions (like heat, cold, oxidative, nitrosative stress, and antibiotic treatment) by altering their gene expression.
- A systems biology approach was used to analyze existing transcriptomic data, revealing that energy conservation leads to the downregulation of certain metabolic pathways while genes related to survival and stress response are upregulated.
- The research highlights 15 genes with unknown functions that are notably upregulated during stress, suggesting they could be important for developing strategies to combat antibiotic resistance.
Background: The recently emerged SARS-CoV-2 caused a global pandemic since the last two years. The urgent need to control the spread of the virus and rapid application of the suitable health measures raised the importance of available, rapid, and accurate diagnostic approaches.
Objective: The purpose of this study is to describe a rapid in-house optimized ELISA based on the expression of the receptor binding domain (RBD) of the SARS-CoV-2 spike protein in a prokaryotic system.
Background: Sodium glucose co-transporter 2 inhibitor (SGLT2i) is a new arment in the prevention and treatment of diabetic kidney disease with a potential effect on reducing and preventing Chronic Kidney Disease (CKD) progression.
Objective: To evaluate the effect of SGLT2 inhibitor in comparison to traditional medication in diabetic patients with microalbuminuria.
Methods: A total of 60 diabetic patients with microalbuminuria were divided into group I, where 30 patients were treated by traditional medications (RAAS blockers) and group II where 30 patients were treated by Dapagliflozin added to the traditional medications.
In enterobacteria, the Rcs system (Regulator of capsule synthesis) monitors envelope integrity and induces a stress response when damages occur in the outer membrane or in the peptidoglycan layer. Built around a two-component system, Rcs controls gene expression via a cascade of phosphoryl transfer reactions. Being particularly complex, Rcs also involves the outer membrane lipoprotein RcsF and the inner membrane essential protein IgaA (Intracellular growth attenuator).
View Article and Find Full Text PDFArticle Synopsis
- A study evaluated the effectiveness of topical nepafenac eye drops in reducing macular edema after panretinal photocoagulation (PRP) in patients with diabetic retinopathy.
- Sixty patients were divided into two groups: one received nepafenac drops, while the other received a control treatment, with results tracked over 6 months.
- The results showed that the nepafenac group had significantly better visual acuity and less central foveal thickness increase compared to the control group, indicating that nepafenac is beneficial for maintaining vision post-PRP.
. To examine causes as well as extent of delay in diagnosis and treatment of primary open angle glaucoma patients in a sample of Egyptians. .
View Article and Find Full Text PDFPurpose. To study factors affecting patients' compliance to antiglaucoma medications in Egypt where there are unique factors as a developing country. Patients and Methods.
View Article and Find Full Text PDFInfestation of cattle by ticks of Rhipicephalus spp. results in severe veterinary and economical losses. Identification of novel proteins from tick salivary glands will enhance our understanding of several aspects of tick physiology and will aid in the development of anti-tick vaccines.
View Article and Find Full Text PDFThe control of Rhipicephalus annulatus ticks in Egypt and other countries relies principally on the application of acaricides which have many drawbacks. Recently, cattle vaccination against ticks showed a potential unconventional approach to control ticks. As a target, salivary glands contain various proteins that may play specific roles during attachment, feeding and may modulate the immune system of the host.
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- Scientists studied a special type of tick called Rhipicephalus annulatus and found a new protein they named Ra-sHSPII by using antibodies from rabbits.
- This protein can help protect other proteins from getting damaged by heat, acting like a shield.
- The researchers found that Ra-sHSPII reacts well with different tick proteins, showing it might be useful for understanding how ticks survive and protect themselves.