Transcriptome-wide identification and computational insights into protein modeling and docking of CAMTA transcription factors in Eleusine coracana L (finger millet).

Calmodulin-binding transcription activator (CAMTA) are a group of transcription factors that are known to perform various important biological functions in plants. Here, we report 7 putative CAMTA transcription factors identified from finger millet transcriptome data. They were further analyzed for physicochemical properties, subcellular localization, conserved domains and motifs, Gene Ontology (GO) terms, phylogeny, 3D structure prediction and CAMTA-Ca-Calmodulin interaction through protein-protein docking.

Computational Identification of MicroRNAs and Their Targets from Finger Millet (Eleusine coracana).

MicroRNAs are endogenous small RNAs regulating intrinsic normal growth and development of plant. Discovering miRNAs, their targets and further inferring their functions had become routine process to comprehend the normal biological processes of miRNAs and their roles in plant development. In this study, we used homology-based analysis with available expressed sequence tag of finger millet (Eleusine coracana) to predict conserved miRNAs.

Identification of microRNAs and their targets in Finger millet by high throughput sequencing.

MicroRNAs are short non-coding RNAs which play an important role in regulating gene expression by mRNA cleavage or by translational repression. The majority of identified miRNAs were evolutionarily conserved; however, others expressed in a species-specific manner. Finger millet is an important cereal crop; nonetheless, no practical information is available on microRNAs to date.

Identification and Characterization of High Temperature Stress Responsive Novel miRNAs in French Bean (Phaseolus vulgaris).

MicroRNAs are important gene regulators controlling almost all biological and metabolic functions. They elicit their regulatory response through modulation of their target gene expression. In this study, we identified eight novel microRNAs (miRNAs) belonging to four miRNA families and one miR* sequence from the French bean genome which responded to high temperature.