Characterization of yellowfin tuna (Thunnus albacares, Scombroidei) tryptophan hydroxylase.

Tryptophan hydroxylase (EC 1.14, 16.4) was purified from yellowfin tuna liver and properties of this enzyme were compared with those of tryptophan hydroxylase from some other species (mouse mastocytoma and rat brain-stem).

Two kinds of neutral serine proteinases in salted muscle of anchovy, Engraulis japonica.

Two kinds of proteinases, type-I and type-II, were purified or partially purified from salted muscle of anchovy, Engraulis japonica. Mol. wts.

Cloning and expression of a chitinase gene from Aeromonas hydrophila in Escherichia coli.

An extracellular secreted chitinase gene from Aeromonas hydrophila was cloned in Escherichia coli, and the gene product was detected in the culture medium. Like the natural chitinase protein, the excreted chitinase had a molecular weight of approximately 85,000 and was subject to catabolite repression by glucose.

Liver 3-hydroxyanthranilic acid oxygenase activity in rainbow trout (Oncorhynchus mykiss).

1. In rainbow trout, 3HAA activity was comparable with those of terrestrial animals; 3HAA:PC activity ratio suggests ineffective conversion of tryptophan to niacin. 2.

Inhibition studies on liver arylformamidases of rainbow trout and cattle.

The effects of L-tryptophan, L-kynurenine, 3-hydroxy-L-kynurenine, ascorbate, some amino acids, 5-hydroxy-L-tryptophan, anthranilate, sodium bisulfite, EDTA, divalent ions and ionic strength on purified liver arylformamidases of rainbow trout and cattle were investigated.

Purification and characterization of liver arylformamidase in rainbow trout and cattle.

1. Arylformamidases were purified from the liver of rainbow trout and cattle. 2.

Enzymatic properties of fish muscle aldolase.

1. Aldolases were isolated from the ordinary muscle of red sea bream Pagrus major, Pacific mackerel Scomber japonicus, and carp Cyprinus carpio by ammonium sulfate fractionation, followed by ion-exchange chromatography on DEAE-cellulose and CM-Sepharose CL-6B columns, and examined for enzymatic properties. 2.

Enzymatic properties of glyceraldehyde-3-phosphate dehydrogenase from fish muscle.

1. Glyceraldehyde-3-phosphate dehydrogenase was isolated from the ordinary muscle of red sea bream Pagrus major, Pacific mackerel Scomber japonicus and carp Cyprinus carpio by ammonium sulfate fractionation, followed by DEAE-Sepharose CL-6B and DEAE-cellulose column chromatography and Sephadex G-150 gel filtration, and examined for enzymatic properties. 2.

A hexokinase from fish liver with wide specificity for nucleotides as phosphoryl donor.

The liver of rainbow trout contains two hexokinases (ATP:D-hexose 6-phosphotransferase, EC 2.7.1.