Comprehensive Laboratory Evaluation of a Lateral Flow Assay for the Detection of .

We conducted a comprehensive, multiphase laboratory evaluation of the Plague BioThreat Alert (BTA) test, a lateral flow immunoassay (LFA), for the rapid detection of . The study was conducted in 7 phases at 2 sites to assess the performance of the LFA. The limit of detection (LOD) was determined using both a virulent and avirulent strain of , CO99-3015 (10 CFU/ml) and A1122 (10 CFU/ml), respectively.

Rapid Presumptive Identification of Isolates Using the Tetracore RedLine Alert™ Test.

A comprehensive laboratory evaluation of the Tetracore RedLine Alert test, a lateral flow immunoassay (LFA) for the rapid presumptive identification of was conducted at 2 different test sites. The study evaluated the sensitivity of this assay using 16 diverse strains of grown on sheep blood agar (SBA) plates. In addition, 83 clinically relevant microorganisms were tested to assess the specificity of the RedLine Alert test.