DESIGN OF IMMUNORADIOMETRIC ASSAY FOR GONADOTROPINS USING A COMMON DETECTION ANTIBODY: AN EXPERIENCE.

We share our experience in the development of immunoradiometric assay (IRMA) for gonadotropins, FSH and LH using common tracer concept. Commercially procured, monoclonal antibody against 'common epitope' of FSH and LH was radiolabeled and epitope specific monoclonal antibody against β-subunit of FSH and LH was chemically immobilized and used as a capture system. In an inclusive assay procedure developed, 100 µl of respective standard and specific capture antibody was incubated with 100 µl common tracer, for 3 h at RT with shaking.

Isolation and characterization of luciferase from Indian firefly, Luciola praeusta.

Luciferase from Indian firefly Luciola praeusta (Coleoptera: Lampyridae: Luciolinae) was isolated and the properties compared with that of the North American firefly, Photinus pyralis. Luciola praeusta luciferase was purified using acetone extraction, gel-filtration column chromatography, ammonium sulfate precipitation and anion exchange chromatography. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis indicates a homogeneous preparation and the molecular mass was slightly higher than that of Photinus pyralis.

Transdermal iontophoresis of insulin. Part 1: A study on the issues associated with the use of platinum electrodes on rat skin.

We have studied the issues associated with the use of platinum electrodes for transdermal iontophoretic delivery of peptides, using insulin as a model peptide. Insulin permeation was studied using full-thickness rat skin by varying the donor solution pH as a function of electrode polarity. The stability of insulin under the iontophoretic conditions was studied using TLC, SDS-polyacrylamide gel electrophoresis and HPLC.