Publications by authors named "Nagaki Ohnuki"
Unlabelled: Enzootic bovine leukosis (EBL), although eradicated in some European countries, is still the most common neoplastic disease of cattle, caused by the bovine leukemia virus (BLV). During the progression of EBL, BLV-infected cells clonally expand, and some of which result in tumor onset. The clonality of BLV-infected cells is generally evaluated with NGS or Sanger sequencing.
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- Bovine leukemia virus (BLV) causes the growth of infected B lymphocytes and up to 5% of infected cows can develop enzootic bovine leukosis (EBL), which involves tumor cell proliferation.
- Using viral integration site analysis through a DNA capture-sequencing method, researchers discovered that identical tumor clones can be found in both blood and tumor tissue, suggesting that these cancer cells can circulate within the bodies of EBL-infected cattle.
- A new method called rapid amplification of integration sites (BLV-RAIS) was developed to efficiently diagnose EBL by detecting clonal expansion of BLV-infected cells, showing high diagnostic sensitivity and specificity, thus providing a reliable tool for early detection of EBL
Article Synopsis
- Bovine leukemia virus (BLV) causes enzootic bovine leukosis (EBL) and has a complicated relationship with the host genome, which isn't fully understood yet.
- A new DNA-capture-seq technique was developed to better understand how BLV integrates into the host genome and to identify clonal expansions of infected cells.
- The study found that BLV integration is prevalent in EBL tumor samples, highlighting the potential of this method for revealing the mechanisms behind BLV infection and its progression in cattle.
Bovine leukemia virus (BLV) is a causative agent of enzootic bovine lymphoma (EBL). BLV is prevalent worldwide, and ten genotypes have been classified based on the sequence of the envelope glycoprotein (gp51) gene. In this study, we present a simple and generally applicable PCR restriction fragment length polymorphism (PCR-RFLP) method to identify BLV genotypes.
View Article and Find Full Text PDFBovine leukemia virus (BLV) belongs to the genus Deltaretrovirus in the family Retroviridae, and is etiologically associated with Enzootic Bovine Leukosis (EBL). The majority of BLV-infected cattle remain asymptomatic throughout their productive lives, whereas approximately 5%-10% of infected cattle develop EBL. Data accumulated recently indicate that whole blood proviral load (PVL) levels of BLV-infected cattle could be an indicator of disease progression in the field.
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