Improved amplification results following episodes of failure to amplify at the Amelogenin Locus using PowerPlex ESI 16 Fast System.

In 2012 the Israel Police DNA Casework laboratory adopted the 16 STR PowerPlex ESI kit for routine use. The Promega Company updated this kit and developed the PowerPlex ESI 16 Fast System in which all autosomal primer pairs remained identical to the original set, except at the amelogenin site. The master mix was improved and optimized which allowed for direct, faster and more robust amplification.

Recognizing the advantage of employing the PowerPlex(®) ESI and PowerPlex(®) ESX complementary kits for purposes of clarification in routine casework.

In an effort to promote European cross-border cooperation in fighting crime and international terrorism the Treaty of Prüm was drafted and accepted within the European forensic community. This move led to the commercial development of new multiplex kits which introduced five new STR loci and promised better performance. Recently the Israel Police DNA Casework and Database laboratories adopted the PowerPlex(®) ESI kit for routine use in our laboratories.