Nitrate removal from industrial wastewater using six newly isolated strains of aerobic heterotrophic denitrifiers in an attached growth.

Background: The objective of this study was to isolate specific heterotrophic aerobic denitrifying bacteria from a wastewater treatment plant and employ them in an attached growth system for wastewater denitrification.

Methods: To isolate and screen aerobic denitrifiers, Denitrifying Medium (DM) and Screen Medium (GN) were utilized. The Polymerase Chain Reaction (PCR) technique and 16S rDNA sequencing were used to identify the isolates.

Isolation, characterization, and effectiveness of bacteriophage Pɸ-Bw-Ab against XDR isolated from nosocomial burn wound infection.

Objectives: With emergence of drug resistance, novel approaches such as phage therapy for treatment of bacterial infections have received significant attention. The purpose of this study was to isolate and identify effective bacteriophages on extremely drug-resistant (XDR) bacteria isolated from burn wounds.

Materials And Methods: Pathogenic bacteria were isolated from hospitalized patient wounds in specialized burn hospitals in Iran, and their identification was performed based on biochemical testing and sequencing of the gene encoding 16S rRNA.

Efficacious antibacterial potency of novel bacteriophages against ESBL-producing isolated from burn wound infections.

Background And Objectives: Prevalence of extended spectrum β-lactamase (ESBL) leads to the development of antibiotic resistance and mortality in burn patients. One of the alternative strategies for controlling ESBL bacterial infections is clinical trials of bacteriophage therapy. The aim of this study was to isolate and characterize specific bacteriophages against ESBL-producing in patients with burn ulcers.

Bacteriophages PɸEn-CL and PɸEn-HO can eliminate MDR Enterobacter cloacae and Enterobacter hormaechei isolated from burn wound infections without toxicity for human skin cells.

The prevalence of multidrug-resistant (MDR) strains has caused serious problems in the treatment of burn infections. MDR Enterobactercloacae and Enterobacterhormaechei have been defined as the causative agents of nosocomial infections in burn patients. In this situation, examination of phages side effects on human cell lines before any investigation on human or animal that can provide beneficial information about the safety of isolated phages.

Using autochthonous as a predatory bacterium for reduction of Gram-negative pathogenic bacteria in urban wastewater and reuse it.

Background And Objectives: The microbial contamination of wastewater is associated with health risks. The aim of this study was to use the autochthonous potential to prey Gram-negative pathogenic bacteria as a bio-control agent to treat urban wastewater.

Materials And Methods: Thirty-six raw sewage samples were collected for isolation of .

The Effect of Environmental Stresses on Gene Expression in Pathogenic spp. through Real-Time PCR.

Leptospirosis is a worldwide infectious and zoonotic disease. The incidence of this disease is high in temperate regions, especially in northern Iran. The aim of this study was to investigate the effects of temperature, pH, and plant extract on the gene expression in pathogenic spp.

Production of glycolipid biosurfactant during crude oil degradation by the novel indigenous isolated Achromobacter kerstersii LMG3441.

This study aimed to find biosurfactant producing and crude oil-degrading bacteria able to decontaminate crude oil from wastewater. The bacteria that were isolated from contaminated sites in an oil refinery plant in Isfahan, Iran, were identified by 16S rDNA sequencing as Achromobacter kerstersii strain LMG3441, Klebsiella pneumonia strain SKBA6, and Klebsiella variicola strain SKV2. According to the results obtained from different tests for the production of biosurfactant among three strains, only Achromobacter kerstersii strain LMG3441 was selected for further study.