The influence of the morphology of titania and hydroxyapatite on the proliferation and osteogenic differentiation of human mesenchymal stem cells.

Herein, the proliferation and osteogenic potential of human mesenchymal stem cells (hMSCs) on the disordered and ordered porous morphology of the titania surface and titania surface modified by hydroxyapatite (HA) are compared for the first time. In 5 days, the MTT-assay showed that the ordered porous morphology of electrochemically fabricated titania nanotubes (TNT) and TNT with chemically deposited hydroxyapatite (TNT-HA) was favorable for stem cell proliferation. In 14 days, RT-qPCR demonstrated that the disordered porous morphology of the sonochemically produced titania mesoporous surface (TMS) and TMS modified by the chemical deposition of HA (TMS-HA) led to the differentiation of hMSCs into the osteogenic direction in the absence of osteogenic inductors.

Characterization of transient cavitation activity during sonochemical modification of magnesium particles.

Investigation of the cavitation activity during ultrasonic treatment of magnesium particles during nanostructuring has been performed. Cavitation activity is recorded in the continuous mode after switching the ultrasound on with the use of ICA-5DM cavitometer. It has been demonstrated that this characteristic of the cavitation zone may be varied in a wide range of constant output parameters of the generator.

Oscillating of physicochemical and biological properties of metal particles on their sonochemical treatment.

Different metal particles are increasingly used to target bacteria as an alternative to antibiotics. Despite numerous data about treating bacterial infections, the utilization of metal particles in antibacterial coatings for implantable devices and medicinal materials promoting wound healing. The antibacterial mechanisms of nanoscale and microscale particles are poorly understood, but the currently accepted mechanisms include oxidative stress induction, metal ion release, and non-oxidative mechanisms.

Sonochemical nanostructuring of titanium for regulation of human mesenchymal stem cells behavior for implant development.

The influence of surface nanotopography of sonochemically generated mesoporous titania coatings (TMS) on the adhesion, proliferation, and osteogenic differentiation of human mesenchymal stem cells (hMSCs) have been investigated in vitro for the first time. It has been revealed that adhesion and proliferation of hMSCs is higher on disordered TMS surfaces compared to smooth polished titania surface after five days of incubation. Surprisingly, the sonochemically generated disordered nanotopography induces the differentiation of hMSCs into osteogenic direction in the absence of osteogenic medium in 14 days of incubation.

Microbubbles trigger oscillation of crystal size in solids.

An understanding of the nature and conditions of nonlinear processes in open systems is important for modulation of the microstructure of solids at a new level of complexity. We demonstrate that cavitation generated by high intensity ultrasound (HIUS) triggers nonlinear processes in microparticles and layers of titanium. We reveal a non-monotonic dependence of the size of grains in the treated solids on sonication time, and oscillation of titanium grain sizes vs.

Switching the Stiffness of Polyelectrolyte Assembly by Light to Control Behavior of Supported Cells.

Polyelectrolyte block copolymer micelles assembled thin film is switched in response to local photocatalytic reactions on titanium dioxide, resulting in a layer of variable height, stiffness in response to visible light irradiation. Preosteoblasts migrate toward stiffer side of the substrates.