Ischemia in pelvic organs as an independent pathogenic factor in the development of benign prostatic hyperplasia and urinary bladder dysfunction.

Blood supply to the pelvic organs of outbred male rats was diminished by graduated constriction of the distal part of the inferior vena cava. Deficiency of intramural blood supply in prostate and urinary bladder was revealed by bioimpedance harmonic analysis according to the magnitude of first cardiac peak in the bioimpedance spectrogram. In 1-1.

[Biological aspects of choice of the intestinal segment for the cystoplasty].

Contractile activity of the iliac and sigmoid intestines versus detrusor activity, reabsorption and secretory activity of the iliac and sigmoid intestinal mucosa in contact with urine were studied in 30 rats. It was found that isolated segments of the iliac and sigmoid intestines have spontaneous contractile activity (stronger in the iliac intestine) while bladder segment contracted only in response to electric stimulation. A contraction-stimulating effect of acetylcholine and a relaxing effect of noradrenaline in experiments with the iliac intestine were close to their effects on the detrusor.

Experimental intravenous cell therapy of acute and chronic renal failure.

The therapeutic effect of intravenous injection of human fetal bone marrow mesenchymal stem cells or summary culture of kidney cells were studied on models of chronic or acute renal failure in outbred albino rats. Both cell types promoted improvement and normalization of the renal function in rats with stable chronic renal insufficiency (2 weeks after kidney cell injection, 1 month after bone marrow mesenchymal stem cell injection). Renal function remained normal or subnormal during the delayed period (3-3.

[Effects of alpha-tocopherol on partial functions of the ischemic kidney].

The experiments on 45 rats and 20 rabbits were made to investigate antiischemic efficacy of alpha-tocopherol in respect of various partial functions of the kidneys compared to its antioxidant activity. It is demonstrated that administration of alpha-tocopherol prior to 30-min heat ischemia arrests activation of membrane lipid peroxidation (LPO) in the tissue of ischemic kidney both in rabbits and rats. Rabbit filtration renal function 1 hour after circulation recovery improved insignificantly, 24 hours after the recovery no difference with the control was registered, while secretory and reabsorption functions of the renal tubules under conditions of pretreatment with alpha-tocopherol significantly improved both in rabbits and rats both 1 hour and 24 hours after ischemia.

Functional aftereffects of intraparenchymatous injection of human fetal stem and progenitor cells to rats with chronic and acute renal failure.

Chronic renal insufficiency was modeled in rats by unilateral nephrectomy and electrocoagulation of both poles of the remaining kidney; acute renal failure was induced by 90-min clamping of the vascular pedicle of the only kidney. Injection of unfractionated culture of human fetal kidney cells or bone marrow mesenchymal stem cells into damaged kidney restored its function in rats with chronic renal insufficiency (observation period up to 2 months). After 2.

[Dynamics of humoral immune response to Treponema pallidum proteins p17 and p41 at early stages of syphilis].

In 60 blood sera from syphilis patients the titers of IgG to T. pallidum antigens p17 and p41 were detected with the use of the test system based on the recombinant analogues of T. pallidum proteins.

[Absence of a correlation between anti-oxidative and anti-ischemic effect of alpha-tocopherol in heat ischemia of the kidney].

Experiments were conducted on 110 inbred albino rats to compare the ability of alpha-tocopherol (alpha-TP), injected in a dose of 200 or 50 mg/kg 3, 6 or 24 hours before the experiment, to inhibit lipid peroxidation (LPO) in the cortex of a kidney after 90-minute ischemia with the influence of the drug on the function of the organ in the early postischemic period. Ischemia and alpha-TP had a mild effect on the basal malonic dialdehyde content in the kidney. Study of ascorbate-induced LPO showed it to be significantly activated in ischemia and after reperfusion, alpha-TP was also found to produce a marked inhibiting effect which grew with the gradual increase of the period between the injection of the agent and the development of ischemia.

[The reasons for the inefficiency of local kidney hypothermia during the temporary discontinuation of their blood supply].

Functional changes in the kidney exposed to 1-hour ischemia with surface cooling to +5+7 degrees C (series I) or +16+18 degrees C (series II) were studied in experiments on 50 rabbits and 20 non-inbred rats. In series II animals significant changes in renal function were not registered. In series I cooling there was a marked decrease of creatinine clearance, clearance of osmotic substances and Na-reabsorption during 1-3 days after ischemia.