Publications by authors named "Nadjla N F Souza"
Article Synopsis
- A PCR protocol was developed for quickly detecting meningococcal DNA in cerebrospinal fluid (CSF), using primers designed from the nspA gene specific to Neisseria meningitidis.
- Testing on 85 N. meningitidis strains and 112 CSF samples showed that nspA-PCR didn't amplify DNA from other bacteria, ensuring specificity for N. meningitidis, with consistent results across different serogroups.
- The method demonstrated high sensitivity, confirming all positive cases from traditional methods, and identifying additional positives that were missed by conventional testing, making it effective even after antibiotic treatment.