Publications by authors named "Nadine Perrot"
Carbapenem resistant (CRAB) represents one of the most challenging pathogens in clinical settings. Colistin is routinely used for treatment of infections by this pathogen, but increasing colistin resistance has been reported. We obtained 122 CRAB isolates from nine Greek hospitals between 2015 and 2017, and those colistin resistant (ColR; = 40, 32.
View Article and Find Full Text PDFSepsis is the leading cause of death among patients in intensive care units (ICUs) requiring an early diagnosis to introduce efficient therapeutic intervention. Rapid identification (ID) of a causative pathogen is key to guide directed antimicrobial selection and was recently shown to reduce hospitalization length in ICUs. Direct processing of positive blood cultures by MALDI-TOF MS technology is one of the several currently available tools used to generate rapid microbial ID.
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- - Mass spectrometry (MS) using Selected Reaction Monitoring (SRM) mode is suggested for detailed analysis of microorganisms in a multiplex format.
- - The SRM method can identify microbes, detect antibiotic resistance, assess virulence, and provide epidemiological typing, all within 60-80 minutes.
- - An example of this approach is presented through the analysis of Staphylococcus aureus, showing its potential for quick characterization of bacteria in sepsis patients' blood cultures.
Matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry (MS) represents a revolution in routine pathogen identification in clinical microbiology laboratories. A MALDI-TOF MS was introduced to tropical Africa in the clinical microbiology laboratory of the Hôpital Principal de Dakar (Senegal) and used for routine pathogen identification. Using MS, 2,429 bacteria and fungi isolated from patients were directly assayed, leading to the identification of 2,082 bacteria (85.
View Article and Find Full Text PDFBiting midges of the genus Culicoides are implicated as vectors for a wide variety of pathogens. The morphological identification of these arthropods may be difficult because of a lack of detailed investigation of taxonomy for this species in Africa. However, matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) profiling is efficient for arthropod identification at the species level.
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- Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry is now commonly used in clinical labs for identifying bacterial species from isolated colonies but struggles with direct identification from clinical specimens due to sample complexity and mixed microbial populations.
- The article presents a new method using penalized non-negative linear regression to analyze single mass spectra, allowing for the identification of species within polymicrobial samples by leveraging existing reference databases.
- Testing on a large set of mono- and bi-microbial samples showed high accuracy, with only 5.3% of cases misidentified, suggesting this approach could enhance routine clinical microbiology practices.