Capturing the Asc1p/eceptor for ctivated inase (RACK1) Microenvironment at the Head Region of the 40S Ribosome with Quantitative BioID in Yeast.

The Asc1 protein of is a scaffold protein at the head region of ribosomal 40S that links mRNA translation to cellular signaling. In this study, proteins that colocalize with Asc1p were identified with proximity-dependent tin entification (BioID), an labeling technique described here for the first time for yeast. Biotinylated Asc1p-birA*-proximal proteins were identified and quantitatively verified against controls applying SILAC and mass spectrometry.