Detection of viable Mycobacterium avium subsp. paratuberculosis in retail pasteurized whole milk by two culture methods and PCR.

Cattle with Johne's disease can shed live Mycobacterium avium subsp. paratuberculosis (MAP) in their milk, and MAP can survive under simulated commercial pasteurization conditions. In several studies conducted in the United Kingdom and Canada, MAP DNA has been detected in retail pasteurized milk samples; however, in one study in the United Kingdom viable MAP was identified in commercially pasteurized milk.

EZ Coli Rapid Detection System: A Rapid Method for the Detection of Escherichia coli O157 in Meat and Other Foods.

The EZ Coli™ Rapid Detection System consists of a selective enrichment medium and a rapid immunological detection kit. After being incubated for 15 to 24 h at 40 to 42°C, an Escherichia coli O157 culture was at a sufficient cell concentration (> 10 CFU/ml) to be tested with the EZ Coli Detection Kit. In studies of foods seeded with E.

Evaluation of Stabilized Bismuth Sulfite Agar for Detection of Salmonella in Foods.

The combination of a new manufacturing process, packaging, and media engineering has all contributed to the development of extended shelf-life (stabilized)-plated media (DuraPak). One of the first products stabilized by this process was bismuth sulfite agar plates (BSA). Stabilized BSA were stored 3-4 months at 35°C and then were inoculated in parallel with lab-prepared BSA following overnight aging at room temperature.