Consumption of Boiled, but Not Grilled, Roasted, or Barbecued Beef Modifies the Urinary Metabolite Profiles in Rats.

Scope: The consumption of processed meat is associated with increased risk of chronic diseases, but determining how the exposure to specific cooking processes alters the metabolome is an analytical challenge. This study aims to evaluate the impact of four typical cooking methods for beef (boiling, barbecuing, grilling, and roasting) on the urinary metabolite profiles in rats, using a non-targeted approach.

Methods And Results: Male Wistar rats (n  =  48) are fed for 3 weeks with experimental diets containing either raw or cooked (boiled, barbecued, grilled, and roasted) beef.

Urinary Medium-Chained Acyl-Carnitines Sign High Caloric Intake whereas Short-Chained Acyl-Carnitines Sign High -Protein Diet within a High-Fat, Hypercaloric Diet in a Randomized Crossover Design Dietary Trial.

The western dietary pattern is known for its frequent meals rich in saturated fat and protein, resulting in a postprandial state for a large part of the day. Therefore, our aim was to investigate the postprandial glucose and lipid metabolism in response to high (HP) or normal (NP) protein, high-fat hypercaloric diet and to identify early biomarkers of protein intake and hepatic lipid accumulation. In a crossover design, 17 healthy subjects were randomly assigned to consume a HP or NP hypercaloric diet for two weeks.

Tissue-specific effect of colitis on protein synthesis in mice: impact of the dietary protein content.

Purpose: Inflammatory bowel diseases are associated with an increase in the whole-body protein turnover, thus possibly requiring an additional supply of dietary proteins. Our aim was to evaluate whether increasing dietary protein content could alleviate protein metabolism alterations in the injured splanchnic and peripheral tissues during colitis and spontaneous mucosal healing.

Methods: Mice with acute chemically induced colitis received either a normal protein (P14, 14% as energy), a moderately (P30, 30%) and a very high-protein (P53, 55%) diets.

Blood 15N:13C Enrichment Ratios Are Proportional to the Ingested Quantity of Protein with the Dual-Tracer Approach for Determining Amino Acid Bioavailability in Humans.

Background: Assessment of amino acid bioavailability is of key importance for the evaluation of protein quality; however, measuring ileal digestibility of dietary proteins in humans is challenging. Therefore, a less-invasive dual stable isotope tracer approach was developed.

Objective: We aimed to test the assumption that the 15N:13C enrichment ratio in the blood increases proportionally to the quantity ingested by applying different quantities of 15N test protein.

Time course of fractional gluconeogenesis after meat ingestion in healthy adults: a DO study.

In the postprandial state, glucose homeostasis is challenged by macronutrient intake, including proteins that trigger insulin secretion and provide glucose precursors. However, little is known about the postprandial response of gluconeogenesis to a protein meal. We aimed to quantify the evolution of fractional gluconeogenesis after a meat meal.

Urinary Metabolomics Profiles Associated to Bovine Meat Ingestion in Humans.

Scope: The impact of meat consumption on human health is widely examined in nutritional epidemiological studies, especially due to the connection between the consumption of red and processed meat and the risk of colon cancer. Food questionnaires do not assess the exposure to different methods of meat cooking. This study aimed to identify biomarkers of the acute ingestion of bovine meat cooked with two different processes.

Biosynthesis of benzylisoquinoline alkaloids in Corydalis bracteata: compartmentation and seasonal dynamics.

Numerous species of the genus Corydalis (Papaveraceae) produce a large spectrum of benzylisoquinoline alkaloids (BIA), some of which are of potential therapeutic value, but no information on sites of their biosynthesis and compartmentation is available. This study focuses on the biosynthesis, compartmentation and seasonal dynamics of BIA in Corydalis bracteata (Steph. ex Willd) Pers.

The Role of Temperature in the Growth and Flowering of Geophytes.

Among several naturally occurring environmental factors, temperature is considered to play a predominant role in controlling proper growth and flowering in geophytes. Most of them require a "warm-cold-warm" sequence to complete their annual cycle. The temperature optima for flower meristem induction and the early stages of floral organogenesis vary between nine and 25 °C, followed, in the autumn, by a several-week period of lower temperature (4-9 °C), which enables stem elongation and anthesis.