Publications by authors named "Nadege Henin"
Unlabelled: Antimicrobial susceptibility testing (AST) of human mycoplasmas using microdilution is time-consuming. In this study, we compared the performance of MICRONAUT-S plates (Biocentric-Bruker) designed for AST of , , and with the results using the Clinical & Laboratory Standards Institute (CLSI) reference method. Then, we investigated the prevalence and mechanisms of resistance to tetracyclines, fluoroquinolones, and macrolides in France in 2020 and 2021.
View Article and Find Full Text PDFArticle Synopsis
- In France, a nationwide survey from 2020 to 2022 reinstated universal LGV testing after switching to selective testing in 2016, focusing on samples from men and women with anorectal positivity.
- The results showed stable LGV positivity rates among men who have sex with men (MSM) but a notable increase in asymptomatic cases, rising from 36.1% in 2020 to 52.4% in 2022.
- The findings emphasize the importance of universal LGV testing and ongoing surveillance due to the increase in asymptomatic cases and changes in genotypes observed in the study.
Article Synopsis
- A multicenter study in France from 2018 to 2020 examined the prevalence of macrolide and fluoroquinolone resistance mutations in patients.
- In metropolitan France, macrolide resistance was found in 34.7% to 42.9% of cases, significantly higher in men compared to women, whereas in overseas France, it was only 6.1% to 14.7%.
- The study concluded that metropolitan France has a notably high prevalence of these mutations compared to overseas regions, with a marked gender disparity in macrolide resistance.
The high prevalence of macrolide resistance in Mycoplasma genitalium results in an increased reliance on moxifloxacin, the second-line treatment; however, moxifloxacin resistance has also emerged. Because assays that can detect fluoroquinolone resistance-associated mutations will be useful for the management of macrolide-resistant M. genitalium infections, we evaluated the performance of three commercial assays (the Allplex MG & MoxiR Assay [Seegene], LightMix Modular parC kit [TIBMOLBIOL], and MGMO qPCR [NYtor) in comparison with gene Sanger sequencing used as the reference.
View Article and Find Full Text PDFIn a previous study, we developed a Multi-Locus VNTRs Analysis (MLVA) typing system, called MLVA-5, for the discrimination of Chlamydia trachomatis genovar E strain. The results suggested the clonal spread of a MLVA-5 type 21 strain among men who have sex with men (MSM). We applied the MLVA-5 typing method on 157 French anorectal genovar E specimens and 19 Swedish specimens collected between 2010 and 2015.
View Article and Find Full Text PDFObjectives: We evaluated the clinical performances of four multiplex real-time PCR commercial kits for the detection of Chlamydia trachomatis, Neisseria gonorrhoeae, Mycoplasma genitalium and Trichomonas vaginalis: the STI PLUS ELITe MGB kit (ELITechGroup), N. gonorrhoeae/C. trachomatis/M.
View Article and Find Full Text PDFArticle Synopsis
- The study assessed how common lymphogranuloma venereum (LGV) is among French men who have sex with men (MSM) using HIV pre-exposure prophylaxis (PrEP), focusing on their clinical and behavioral traits.
- Out of 486 anorectal specimens tested in 2017, 18.7% were positive for LGV, with most cases being symptomatic and associated with higher numbers of sexual partners and concurrent syphilis.
- The study suggests that MSM diagnosed with chlamydia and exhibiting high-risk sexual behaviors should be routinely tested for LGV and treated presumptively, marking the first comprehensive surveillance of LGV in this demographic.
The aim of this study was to evaluate the clinical performance of the Aptima transcription-mediated amplification (MG-TMA) CE-marked for diagnosis (CE-IVD) assay for the detection of in male and female clinical samples in comparison with the in-house real-time PCR (in-house PCR) assay routinely used in our laboratory. A total of 1,431 clinical specimens obtained from 1,235 patients were prospectively collected at the Bacteriology Department of Bordeaux University Hospital (France). Additional research-use-only Aptima transcription-mediated amplification (TMA) assays, Alt1-TMA and Alt2-TMA, were performed on discordant specimens to determine infection status.
View Article and Find Full Text PDFWe describe a change in the molecular epidemiology of Chlamydia trachomatis strains involved in an outbreak of rectal lymphogranuloma venereum in France during January 2010-April 2015. Until 2012, the C. trachomatis L2b strain predominated; however, starting in 2013, most cases involved the L2 strain.
View Article and Find Full Text PDF