The RNA Binding Protein Tristetraprolin Contributes to mRNA Stability in Cystic Fibrosis.

Cystic Fibrosis (CF) is the most common inherited disorder and is characterized by an inflammatory phenotype. Here, we found that in bronchial epithelium reconstituted form lung tissue biopsies from patients with CF, the RNA-binding protein tristetraprolin (TTP), a key regulator of inflammation, is dysregulated in cells that strongly express cytokines and interleukins. TTP activity is regulated by extensive post-translational modifications, particularly phosphorylation.

[Children with psoriasis in secondary care: Clinical aspects and comorbidities diverge from the generally published data].

Background: Psoriasis affects 0.2-0.7 % of children and is associated with obesity.

Early failure of articular surface replacement XL total hip arthroplasty.

The ASR (articular surface replacement) XL (DePuy, Warsaw, Ind) metal-on-metal hip arthroplasty offers the advantage of stability and increased motion. However, an alarming number of early failures prompted the evaluation of patients treated with this system. A prospective study of patients who underwent arthroplasty with the ASR XL system was performed.

In vivo kinematics after a cruciate-substituting TKA.

Unlabelled: Patterns of motion in the native knee show substantial variability. Guided motion prosthetic designs offer stability but may limit natural variability. To assess these limits, we therefore determined the in vivo kinematic patterns for patients having a cruciate-substituting TKA of one design and determined the intersurgeon variability associated with a guided-motion prosthetic design.

New trends and early clinical outcomes with a modern knee revision system.

The incidence of revision total knee arthroplasty (TKA) has grown tremendously the past decade, and all projections suggest that it will continue to increase during the next 25 years. Although primary TKA remains one of the most successful orthopedic procedures, revision TKA has not as well. Failure rates for revision TKA remain significantly higher than those for primary TKA.

Bicompartmental knee arthroplasty: a bone-sparing, ligament-sparing, and minimally invasive alternative for active patients.

In the past, treatment of knee osteoarthritis has been limited to unicompartmental knee replacement or total knee arthroplasty (TKA). Neither option is well suited for the active patient with midstage osteoarthritis of the medial and patellofemoral compartments. Now an alternative treatment is available that targets the diseased area without sacrifice of normal bone or both the cruciate ligaments.

Evaluation of off-loading braces for treatment of unicompartmental knee arthrosis.

Two analyses were performed to assess the effectiveness of off-loading knee braces in producing condylar separation of degenerative compartments in patients with unicompartmental knee osteoarthritis. All patients were analyzed using video fluoroscopy under weight-bearing conditions. In the initial study, a single brace was analyzed in 2 dimensions and demonstrated that medial condylar separation was obtained, with corresponding pain relief, in 78% of patients.

Cementless revision total hip arthroplasty without allograft in severe proximal femoral defects.

This study evaluates the difficult reconstructive challenge of severe proximal femoral bone loss. We present intermediate-term results of 46 hips with extensive proximal femoral bone loss that underwent revision total hip arthroplasty using cementless distal fixation without supplemental allograft. All were evaluated with the Harris hip score at a minimum of 2 years.

Bipolar reconstruction for recurrent instability of the hip.

Between 1986 and 1998, 81 total hip revisions were performed for recurrent instability. Twenty-three revisions were fixed cups to bipolars. Average follow-up was 50 months.

Underestimation of osteolysis in posterior stabilized total knee arthroplasty.

Periprosthetic osteolysis in total knee arthroplasty (TKA) has become a significant problem. Routine surveillance is warranted to detect problems that may not be symptomatic. The goal of these radiographs is to detect arthroplasty-related bone loss at an early stage to implement strategies to limit its extension.

Early failures in total knee arthroplasty.

Total knee arthroplasty is a predictable operation. Unfortunately, there is a subset of patients who do not do well and require revision surgery within the first 5 years. The purpose of the current study was to analyze the mechanisms of failure in patients who had revision surgery within 5 years of their index arthroplasty.

Do facet joint capsular ligaments contain estrogen receptors? Application to pathogenesis of degenerative spondylolisthesis.

Studies showing that degenerative spondylolisthesis is 4 to 5 times more common in females than in males have suggested that hormonal influences account for this gender difference. Estrogen has been shown to play a role in other instabilities, such as those of the anterior cruciate ligament and the shoulder capsular ligaments, and estrogen receptors have been identified in these tissues. We wanted to assess facet joint capsular ligaments for the presence of such receptors.

Sacroiliac joint injection: a cadaveric study.

Eleven bony pelves were studied in an attempt to find an ideal approach for needle placement into the sacroiliac joint and to describe the unique anatomy of the sacroiliac joint relative to sacroiliac joint injection. A posterior approach starting 2 cm to 3 cm inferior to the posterior superior iliac spine, angled 20 degrees to 30 degrees laterally, relative to the sagittal plane, and 10 degrees to 20 degrees inferiorly, relative to the transverse plane, was found to be the best approach to the intra-articular portion of the sacroiliac joint.

Morphologic considerations of the first sacral pedicle for iliosacral screw placement.

Study Design: Morphometric, radiographic, and computed tomographic evaluation of the pedicle of the first sacral vertebra was performed, and the pedicle's spatial relation with the posterior surface of the ilium was defined.

Objectives: To facilitate accurate localization of the entry site of the iliosacral pedicular screw on the posterior surface of the ilium, to provide optimal length and direction of iliosacral screw placement, and to investigate the feasibility of inserting two screws through the first sacral vertebral pedicle for unstable posterior pelvic fixation.

Methods: Anterior and posterior pedicular height, pedicular depth, alar depth, and posterior alar height of S1 vertebrae were measured in 11 body pelves bilaterally.

Local tissue of the lumbar spine response to titanium plate--screw system. Case reports.

Study Design: This study reported the histopathologic findings of peri-implant tissue harvested from two patients with titanium plate-screw fixation in the lumbar spine.

Objectives: To describe and discuss the histopathologic findings with regard to local tissue response to the titanium plate-screw system in the lumbar spine.

Summary Of Background Data: There are no reports about local tissue of the lumbar spine response to titanium plate-screw system in the literature.

The location of the cervical nerve roots on the posterior aspect of the cervical spine.

Study Design: This study analyzed anatomic parameters between the midpoint of cervical vertebral lateral masses as seen on the superficial, posterior aspect of the mass and cervical nerve roots. Posterior cervical dissection was performed, with the midpoint of the lateral masses kept intact and the nerve roots exposed.

Objective: To quantitatively determine the location of the cervical nerve roots and the transverse foramina, indicating vertebral artery placement relative to the posterior aspect of the cervical spine.

Morphology of the second cervical vertebra and the posterior projection of the C2 pedicle axis.

Study Design: This study assessed numerous structural features of the second cervical vertebra (C2), describing the projection point of the pedicle on its posterior aspect.

Objectives: Evaluation of the specimens included quantitative description of 18 linear and four angular parameters, which then were correlated between male and female specimens. The point of projection of the C2 pedicle axis was described with an emphasis on a perspective relevant to a posterior approach.

A new scheme for additional typing of P. aeruginosa using antisera prepared against glycopolypeptide derived from the slime of the microorganisms.

The author used the slide agglutination with antisera prepared against glycopolypeptides (GP) derived from the slime of the P. aeruginosa mucoid strains to classify one hundred and nine P. aeruginosa strains in three homogeneous groups.

Effects of a glycopolypeptide from the slime of P. aeruginosa on phagocytosis by mouse macrophages.

The isolation and characterization of a glycopolypeptide (GP) from the slime of Pseudomonas aeruginosa which has a marked effect on phagocytosis of P. aeruginosa cells by mouse macrophages is described. The GP was found to be a polysaccharide coupled with a polypeptide consisting of monomers with a molecular weight of 25,000.

[A micromethod for studying the sensitivity of anaerobic bacteria to antibiotics].

The method described here allows to classify anaerobic bacteria isolated from clinical specimens in four categories of sensitivity or resistance to 8 antibiotics. Previous experiments using eight reference strains led to choose the pre-reduced Schaedler broth supplemented with hemin, vitamin K1, and 0.07 p.

Activity of sulfamethoxazole trimethoprim against Bacteroides fragilis.

The author tested parallely 35 Bacteroides fragilis strains by the agar diffusion method and disk technique. A notable discrepancy appears between these two procedures. No regression line can be constructe and the inhibition diameter measurement is unable to class the strain in "susceptible" or "resistant".

Media supplemented with nalidixic acid for the isolation of gram negative anaerobic bacteria.

The minimum inhibitory concentrations (MIC) of nalidixic acid were determined for thirty-four strains of Clostridium sp. by an agar dilution technique. The MIC range of Clostridium perfringens ran from 0.