HLA alloimmunization in Egyptian aplastic anemia patients receiving exclusively leukoreduced blood components.

Background: The aim of the work was to detect the presence of anti-human leukocyte antigens (anti-HLAs) class I and II antibodies in sera of multitransfused aplastic anemia pediatric patients using two different techniques. The effect of the implemented transfusion practice on the frequency of these antibodies was studied as well as their effect on the patient's clinical condition.

Methods: Flowcytometry panel reactive antibodies (FlowPRAs) for HLA class I and II were determined and compared to the results obtained by Complement-dependent-cytotoxicity (CDC) assay.