Optimization and purification of mannanase produced by an alkaliphilic-thermotolerant N1 isolated from Bani Salama Lake in Wadi El-Natron.

An alkaliphilic-thermotolerant N1 isolated from Bani Salama Lake, Wadi El-Natron, Egypt, was proved to produce mannanase enzyme. Optimization of the fermentation medium components using Plackett-Burman design was applied. Glucose and inoculum size were found to be the most significant factors enhancing the production of the enzyme.