The GTPase Domain of MX2 Interacts with the HIV-1 Capsid, Enabling Its Short Isoform to Moderate Antiviral Restriction.

Myxovirus resistance 2 (MX2/MXB) is an interferon (IFN)-induced HIV-1 restriction factor that inhibits viral nuclear DNA accumulation. The amino-terminal domain of MX2 binds the viral capsid and is essential for inhibition. Using in vitro assembled Capsid-Nucleocapsid (CANC) complexes as a surrogate for the HIV-1 capsid lattice, we reveal that the GTPase (G) domain of MX2 contains a second, independent capsid-binding site.

Endoscopic Management of Pediatric Complex Hydrocephalus.

Objective: To define the role of neuroendoscopy as an adjuvant technique for the management of pediatric complex hydrocephalus.

Methods: We performed a retrospective analysis of a series of pediatric patients who had undergone surgery for complex hydrocephalus from January 2002 to March 2017 at 2 pediatric neurosurgery units in Rome, Italy and Mansoura, Egypt. The endoscopic procedures performed included cyst or membrane fenestration, septum pellucidotomy, foraminoplasty, and aqueductoplasty with or without a stent.

Development of salt and pH-induced solidified floating organic droplets homogeneous liquid-liquid microextraction for extraction of ten pyrethroid insecticides in fresh fruits and fruit juices followed by gas chromatography-mass spectrometry.

A new microextraction method named salt and pH-induced homogeneous liquid-liquid microextraction has been developed in a home-made extraction device for the extraction and preconcentration of some pyrethroid insecticides from different fruit juice samples prior to gas chromatography-mass spectrometry. In the present work, an extraction device made from two parallel glass tubes with different lengths and diameters was used in the microextraction procedure. In this method, a homogeneous solution of a sample solution and an extraction solvent (pivalic acid) was broken by performing an acid-base reaction and the extraction solvent was produced in whole of the solution.

Spontaneous Cerebellar Hematoma: Decision Making in Conscious Adults.

Objective: To detect predictors of the clinical course and outcome of cerebellar hematoma in conscious patients that may help in decision making.

Methods: This study entails retrospective and prospective review and collection of the demographic, clinical, and radiologic data of 92 patients with cerebellar hematoma presented conscious and initially treated conservatively. Primary outcome was deterioration lower than a Glasgow Coma Scale score of 14 and secondary outcome was Glasgow Outcome Scale score at discharge and 3 months later.

Determination of three antidepressants in urine using simultaneous derivatization and temperature-assisted dispersive liquid-liquid microextraction followed by gas chromatography-flame ionization detection.

This paper presents a fast and simple method for the extraction, preconcentration and determination of fluvoxamine, nortriptyline and maprotiline in urine using simultaneous derivatization and temperature-assisted dispersive liquid-liquid microextraction (TA-DLLME) followed by gas chromatography-flame ionization detection (GC-FID). An appropriate mixture of dimethylformamide (disperser solvent), 1,1,2,2-tetrachloroethane (extraction solvent) and acetic anhydride (derivatization agent) was rapidly injected into the heated sample. Then the solution was cooled to room temperature and cloudy solution formed was centrifuged.

Solid-based disperser liquid-liquid microextraction for the preconcentration of phthalate esters and di-(2-ethylhexyl) adipate followed by gas chromatography with flame ionization detection or mass spectrometry.

A new approach for the development of a dispersive liquid-liquid microextraction followed by GC with flame ionization detection was proposed for the determination of phthalate esters and di-(2-ethylhexyl) adipate in aqueous samples. In the proposed method, solid and liquid phases were used as the disperser and extractant, respectively, providing a simple and fast mode for the extraction of the analytes into a small volume of an organic solvent. In this method, microliter levels of an extraction solvent was added onto a sugar cube and it was transferred into the aqueous phase containing the analytes.

A case-control study of an intraoperative corneal abrasion prevention program: holding the gains made with a continuous quality improvement effort.

Background: Corneal injury is the most frequent ocular complication during general anesthesia. Although prevention has appeared feasible, inconsistent use and timing of conventional eye ointment and eyelid tape had failed to adequately prevent intraoperative corneal injuries at a department of anesthesiology in an academic medical center. A continuous quality improvement (CQI) program was thus undertaken to prevent intraoperative corneal injury.

Cytokine gene polymorphisms in egyptian cases with brain tumors.

Background: Cytokines are proposed to play important roles in brain tumor biology as well as neurodegeneration or impaired neuronal function.

Objectives: This work aimed to check the association of polymorphisms of cytokine genes in Egyptian cases with brain tumors.

Methods: This work included 45 cases affected by brain tumors diagnosed as 24 benign and 21 malignant.

Cytokine gene polymorphisms in Egyptian cases with brain tumors.

Cytokines are proposed to play important roles in brain tumor biology as well as neurodegeneration or impaired neuronal function. To evaluate the association of polymorphisms of cytokine genes with brain tumors in Egyptian patients. This study included 45 cases affected by brain tumors.