Acaricidal activity of paste of methanolic extract of leaf of , hybrid, and against the bovine ticks in in-vitro condition.

The objective of this study was to evaluate the acaricidal activity of a paste made from freeze-dried methanolic extracts of air-dried leaf powders of , hybrid, , and against () on cellulose paper. The extracts were tested in both single form (100% and 50% concentration) and dual combination (prepared by mixing equal proportions of the extracts in 100% concentration). The results showed a direct proportional relationship ( < 0.

Loop-mediated isothermal amplification assay for the rapid detection of in cattle based on major surface protein 5 gene.

Background & Objectives: Timely intervention is needed to minimize the economic losses of vector-borne bovine anaplasmosis which can be possible by the isothermal amplification assay.

Methods: Anaplasma marginale in the cattle of south Gujarat, India was detected in the PCR and LAMP by amplifying the fragment of msp5 gene. The PCR product was digested with EcoRI, and sequenced to confirm its pathogen specific detection.