Publications by authors named "N Y Sardesai"
Single-cell and spatial transcriptomics technologies have significantly advanced our understanding of the molecular mechanisms underlying crop biology. This review presents an update on the application of these technologies in crop improvement. The heterogeneity of different cell populations within a tissue plays a crucial role in the coordinated response of an organism to its environment.
View Article and Find Full Text PDFInsulators are -regulatory elements that separate transcriptional units, whereas silencers are elements that repress transcription regardless of their position. In plants, these elements remain largely uncharacterized. Here, we use the massively parallel reporter assay Plant STARR-seq with short fragments of eight large insulators to identify more than 100 fragments that block enhancer activity.
View Article and Find Full Text PDFArticle Synopsis
- PD-1 inhibitors show limited effectiveness on their own for treating hepatocellular carcinoma (HCC), but a new personalized therapeutic cancer vaccine (PTCV) may boost their efficacy by enhancing immune responses against tumors.
- In a study, a DNA plasmid PTCV combined with pembrolizumab was tested on patients with advanced HCC; the treatment was found to be relatively safe with manageable side effects and showed a 30.6% objective response rate.
- The study observed that patients with more neoantigens from the vaccine had better clinical responses, with immune profiling revealing a strong T cell response directed at tumor cells, supporting the vaccine's potential as a viable therapeutic approach.
Background: Low-normal levels of arterial carbon dioxide (PaCO2) are recommended in the acute phase of traumatic brain injury (TBI) to optimize oxygen and CO2 tension, and to maintain cerebral perfusion. End-tidal CO2 (ETCO2) may be used as a surrogate for PaCO2 when arterial sampling is less readily available. ETCO2 may not be an adequate proxy to guide ventilation and the effects on concomitant injury, time, and the impact of ventilatory strategies on the PaCO2-ETCO2 gradient are not well understood.
View Article and Find Full Text PDFPurpose Of The Study: Generative conversational artificial intelligence (AI) has huge potential to improve medical education. This pilot study evaluated the possibility of using a 'no-code' generative AI solution to create 2D and 3D virtual avatars, that trainee doctors can interact with to simulate patient encounters.
Methods: The platform 'Convai' was used to create a virtual patient avatar, with a custom backstory, to test the feasibility of this technique.