[Influence of Lactobacillus fermentum metabolites on ultrastructure of pathogenic Escherichia coli].

Aim: To study ultrastructural changes of hemolytic and enterohemorrhagic Escherichia coli during interaction with metabolites of Lactobacillus fermentum.

Materials And Methods: Strains of pathogenic hemolytic (Hly) and enterohemorrhagic Escherichia coli (O157:H7) as well as symbiotic bacteriocinogenic strain Lactobacillus fermentum 97 were used. Inhibition of growth of viable cells was performed by delayed antagonism method.

[Interaction of Clostridium difficile with bacterial associations of parietal biofilm in colon of mice].

Using electron microscopy, ultrastructural organization of microbial parietal biofilm in colon of immunodeficient mice line B10-hr(rhy) was studied before and after peroral inoculation with enteropathogenic strain of Clostridium difficile. It was shown that infection leads to dispersion of normal biofilm in various sites and imbalance in natural proportions of different bacterial associations. Also, clear ultrastructural signs of involution of Gram-negative microorganisms were observed.

[A comparative study of the role of the yadA, invA, and psaA genes in the pathogenicity of Yersinia pseudotuberculosis].

The roles of yadA, invA, and psaA genes introduced into the genetic background of the Y. pseudotuberculosis strain possessing the large p VM82 plasmid in virulence and invasion capacity were studied. Isogenic single mutants as well as double and multiple mutants of these genes were constructed and used.

[Clinical and morphological characteristics of migrating erythema in patients with Ixodes tick-borne Lyme disease].

Patients with erythematous Ixodes tick-borne relapsing fever were examined and their skin biopsy specimens were morphologically studied to reveal clinical, immunological and morphological features of erythematous Ixodes tick-borne relapsing fever. Two types of development of erythema migrans were identified. These include 1) a typical type that appears as an area of homogenous hyperemia or that of annular shape and 2) an atypical one that presents as minor vesicles.

[Preparation of T-lymphoblastoid cell lines from patients with T-cell leukemia and asymptomatic carriers of HTLV-I: analysis of the viral genome status].

Three HTLV-I-infected partially interleukin-2-dependent lymphoid cell lines were derived from a patient with T-cellular leukemia (ATL) from Georgia and a carrier of HTLV-I from Sakhalin. The strains cultured in the presence of 3-5% interleukin-2 were designated as NBK-1, NBK-2, and YE-1, respectively. Immunoblotting analysis showed typical HTLV-I proteins in them except NBK-2 which expressed nontypical proteins p40K and p28-29K.