Simultaneous sequencing of genetic and epigenetic bases in DNA.

DNA comprises molecular information stored in genetic and epigenetic bases, both of which are vital to our understanding of biology. Most DNA sequencing approaches address either genetics or epigenetics and thus capture incomplete information. Methods widely used to detect epigenetic DNA bases fail to capture common C-to-T mutations or distinguish 5-methylcytosine from 5-hydroxymethylcytosine.

[Effect of bisphosphonates (HEBP, CL2MBP) on the process of biological mineralization in fresh bone and bone tissue de novo as a result of experimental osteogenesis induction in guinea pigs].

It is known from the literature that bisphosphonates inhibit mineralization process in skeletal tissues. To study in vivo the effects of HEBP (etidronate) and Cl2MBP (clodronate) both on preexisting bone mineral as well as on the early stages of the mineralization process the experimental model of heterotopical bone induction was chosen. Urinary bladder mucosa was used as an inductor of osteogenesis.