In Vitro Collection for the Safe Storage of Grapevine Hybrids and Identification of the Presence of Resistance Genes.

This paper focuses on the creation of an in vitro collection of grapevine hybrids from the breeding program of the Kazakh Scientific Research Institute of Fruit Growing and Viticulture and investigates the presence of resistance mediated by and loci. We looked at the optimization of in vitro establishment using either shoots taken directly from field-grown plants or from budwood cuttings forced indoors. We further screened for the presence of endophyte contamination in the initiated explants and optimized the multiplication stage.

In vitro cold acclimation is required for successful cryopreservation of Juglans regia L. shoot tips.

Background: Persian walnut (Juglans regia L.) is one of the most economically important nut crops. In the Western Tien Shan in Kazakhstan, walnut forests are the northernmost in the natural range of this species.

Effect of Plant Preservative Mixture on Endophytic Bacteria Eradication from In Vitro-Grown Apple Shoots.

Endophytic contaminants are a common problem for the in vitro propagation of woody plants and have significant economic repercussions for the conservation of plant genetic resources and commercial micropropagation. In this study, first, the microbial contamination that appeared around the base of in vitro-grown apple shoots was identified as . Then, plant preservative mixture (PPM) was used as a bactericidal agent in plant tissue culture.

CRYOTHERAPY AS A METHOD FOR REDUCING THE VIRUS INFECTION OF APPLES (Malus sp.).

Background: There is an urgent need in Kazakhstan for virus-free nursery stock to reinvigorate the industry and preserve historic cultivars. An in vitro collection of apples could be used for virus testing and elimination and to provide virus-free elite stock plants to nurseries.

Methods: Malus sieversii Ledeb.

Cold acclimation improves regrowth of cryopreserved apple shoot tips.

Cryopreservation is important for safeguarding the genetic resources of apple germplasm in Kazakhstan, the center of origin for apples. In this study, conducted with five apple genotypes [Malus domestica Borkh. and Malus sieversii (Ledeb.