The objective of the present study was to optimize genotyping of nuclear DNA contained in human hair. The most efficient procedures for DNA isolation and typing are described taking into consideration the hair growth phase, the epithelial tissue conditions, and the number of nuclei in the near-root ends of the hair. The recommendations for the expert interpretation of the results of the molecular-genetic investigations are proposed.
View Article and Find Full Text PDFThe objective of the present work was to develop the method for the selection of hairs suitable for the molecular-genetic investigation of nuclear DNA. It was proposed to distinguish between five groups of hair depending on its growth phase, the presence or absence of epithelial tissues at the root terminus. An algorithm was developed for carrying out prognostic screening including the morphological and cytomorphological investigations the results of which can be used to evaluate the possibility for further genotyping.
View Article and Find Full Text PDFThe authors describe a domestically produced test-system for the determination of the AB0 blood type by means of the single nucleotide polymorphisms (SNP) analysis. The results of the trials indicate that the proposed test-system can be employed for the investigation of DNA specimens of individual origin obtained from any objects of expertise including micro-objects containing human nuclear DNA.
View Article and Find Full Text PDFThis article is concerned with the management of the disputable situations arising in the course of establishment of the kinship based on the analysis of autosomal STR loci. It is proposed to enhance the accuracy of determining thekinsip relations in the parent-child pairs (in the absence of one of the parents) by using additional sets of genetic markers localized for example on sex chromosomes, mitochondrial DNA (mtDNA) and bi-allele markers.
View Article and Find Full Text PDFWe compared the effectiveness of DNA isolation from nail plates using three commercial kits from different manufacturers. The results of the study give reason to recommend the use of PrepFiler BTA Forensic DNA Extraction Kits and QIAamp DNA Investigator Kit for the work with nail microfragments.
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