Assembly of the Xrn2/Rat1-Rai1-Rtt103 termination complexes in mesophilic and thermophilic organisms.

The 5'-3' exoribonuclease Xrn2, known as Rat1 in yeasts, terminates mRNA transcription by RNA polymerase II (RNAPII). In the torpedo model of termination, the activity of Xrn2/Rat1 is enhanced by Rai1, which is recruited to the termination site by Rtt103, an adaptor protein binding to the RNAPII C-terminal domain (CTD). The overall architecture of the Xrn2/Rat1-Rai1-Rtt103 complex remains unknown.

[Microsporogenesis and pollen formation in Scotch pine (Pinus sylvestris L.)].

The prolongation of a warm season during autumn, typical for the Siberia in recent 5-6 years, caused changes in the terms of reproductive processes in microsporangia of Scotch pine in the Krasnoyarsk region. The meiosis started in the autumn of the year, preceding the pollination, and comes to the end in next spring, whereas usually this process occurs in the spring of the year of pollination. In such conditions the division of microsporocytes accompanied with different abnormalities, causing various anomalies and the incomplete development of pollen grains.

[Herpes simplex virus type 1 and 2 (HSV-1,2) DNA detection by PCR during genital herpes].

The oligonucleotide primers for herpes simplex virus type 1 and 2 DNA detection are developed. In examined group of patients with genital herpes the virus of type 2 and type 1 was detected in 63% and 26% cases, respectively. The mixed infection of both types is revealed in 11% of the patients.

[Designing of the pp65 recombinant antigen of human cytomegalovirus and investigation of its immunochemical properties].

The primary and secondary structures of the pp65 phosphoprotein of human cytomegalovirus coded by the UL83 gene were studied by the methods of computer-aided analysis. An immunodominant protein fragment with 3 antigenic determinant was detected. The UL83 fragment coding the selected region was amplified and cloned in bacterial expressing vector.

[Efficacy of therapeutic and prophylactic actions of ultralow doses of antibodies to gamma-interferon in experimental murine model of herpes virus].

The process of the disease due to herpes simplex virus types 1 and 2 (HSV-1 and 2) was studied on white uninbred mice weighing 10 to 12 g. The animals were infected intracerebrally or intraperitoneally. Intraperitoneal contamination of the animals with MS strain of HSV-2 was used for the experimental model of the herpes simplex infection.