MOLECULAR GENETIC CHARACTERISTICS OF ALLELE VARIANTS OF MICROSATELLITE LOCI DU281, DU215, AND DU323 IN THE PARTHENOGENETIC LIZARDS DAREVSKIA ROSTOMBEKOVI (FAMILY LACERTIDAE) .

One of the main questions-in the study of the unisexual (parthenogenetic) species of vertebrates is the determination of their genetic diversity. Nuclear and mitochondrial genetic markers can be used for this purpose. One of the most effective genetic markers is the microsatellite DNA, which mutates at a high rate.

[Medical-social support to the immobile ill elderly living in a mega polis].

The article describes the working experience of the medical-social support department of the municipal clinic. The work is based on the cooperation with the department of social security. The article focuses on the reasons of decreasing the function ability of such people and age-related features of immobile elderly people.

[Proteolytic activation of prophenoloxidase in the imago of musca domestica].

The enzyme phenoloxidase complex of insects generally localized in cuticle and hemolymph, presents in the form of inactive proenzymes, which may be actitivated under the influence of a range of enzymes, components of the "phenoloxidase activated system" or "prophenoloxidase cascade". Currently the whole chain of the reactions is not clear, but it includes esterases, which activate serine proteinase, including terminal proteinase, acid phosphatase, some dehydrogenase, and protein kinase. The typhoid fly imago has monophenol-monooxygenase (MPMO) in the form of proenzyme, which is activated under the influence of endogenous and exogenous proteases.

[Specific interaction between esterases and 2-butylthio-2-thio-1,3,2-oxazaphosphorynane and its cyclic and acyclic analogs].

We studied the anticholinesterase and anticarboxylesterase effects of 1,3,2-oxazaphosphorynane derivatives and certain cyclic and acyclic analogs on the two enzymes of homoiotherms (ACE from human erythrocytes and BuCE from horse serum) as well as the enzymes from insect tissues (the nerve cord of the American cock-roach and the cephalic region of the domestic fly). The differences in in vitro antiesterase activity of cyclic thionic and the corresponding oxo derivatives of phosphorinane were revealed. The mechanism of the esterase active center phosphorylation not only splitting off the outgoing group (in vivo) but also opening the cycle by P-O bond (in vitro and possibly in vivo) is usually proposed to explain the higher inhibiting activity of the thionic compounds compared to the oxonic ones.

[Ecdysterone induces the activity of multiple forms of acid phosphatase and malate dehydrogenase in fat bodies of Anterahea pernyi pupae].

The method of electrophoresis in PAAG has been used to study activity of multiple forms of acid phosphatase and malate dehydrogenase of the fat body of Anterahea pernyi pupae in norm and affected by ecdysterone. It is shown that an increase of total activity of acid phosphatase and malate dehydrogenase with administration of a steroid hormone is promoted by growth of activity of certain inducible multiple forms of the studied enzymes. Ecdysterone injection considerably elevates activity of the most electrophoretically mobile form of acid phosphatase and provokes new formation of malate dehydrogenase form.