Formation and some cytophysiological characteristics of polynuclear macrophages in primary cultures of peritoneal cells.

We studied the formation and cytophysiological characteristics of polynuclear macrophages in primary cultures of peritoneal cells from C57Bl/6 mice. Production of reactive oxygen species and phagocytic activity in polynuclear macrophages were higher than in mononuclear macrophages. The formation of polynuclear macrophages in cultures of peritoneal cells is realized via amitosis.

Is the human trophoblast in vitro an adaptive tissue for immunological influences?

The role of the extra-embryonic organs (e.g. placenta) in the feto-maternal immunological interactions is not clear.

[Study of a trophoblast cell culture in vitro].

The authors obtained trophoblast culture from an early chorion of human embryo. Trophoblastic cells of the main 4 types described were: giant cells, with processes, fibro-blast-like, and round. All the cells were rich in RNA, glycoproteins, had a set of enzymes of the Krebs pentose cycle, but low activity of succinic dehydrogenase.

[Capacity for in vitro growth and the character of synovial fluid cell transformation in rheumatoid arthritis patients].

Investigations carried out permitted to detect the in vitro growth of cells in the synovial fluid of patients suffering from rheumatoid arthritis. Five types of cells (macrophages, polykariocytes, fibroblast-like cells histiocytes and lymphocytes) appeared during transformation. It was revealed that the intensity of the cell growth in the culture depended on the activity of the disease; there was also a significant decrease of this intensity (P less than 0.