[Redox reaction of flavin and pyridine nucleotides of Hirudo medicinalis leech nerve cells].

On different Retzius cell preparations (cell in the whole leech, in part of the animal and in isolated ganglionic chain) it has been demonstrated that the process of preparation itself affects redox levels of flavoproteins and pyridine nucleotides in the intact cell, changes their redox response to oxygen and alters the direction of redox reaction of pyridine nucleotide to 5 mM oxaloacetate. Redox levels of flavin and pyridine nucleotides in the cell were evaluated by the intensity of its fluorescense in violet and green parts of the spectrum.