The state of tryptophan-containing sites of human, bovine and rabbit plasminogens with changing solution pHs.

The state of tryptophan-containing sites is proved to be stable by intrinsic tryptophan fluorescence with pH 5-8, 7-9, and 6-9 in human, rabbit and bovine plasminogen molecules, respectively. With pH < 5.0 tryptophan-containing sites of human zymogen (in contrast to rabbit and bovine ones) undergo conformational transitions.

Conformation ability test of human, rabbit and bovine plasminogens and their specific interaction with streptokinase.

Human, rabbit and bovine plasminogens, having different sensitivity to streptokinase-activating action, differ, according to spectrophotometric titration, tryptophan fluorescence and circular dichroism spectroscopy, in the state of tyrosine and tryptophan residues, and in secondary and tertiary structures. Human plasminogen-streptokinase equimolar complex formation (according to gel chromatography) is accompanied by a differential ultraviolet spectrum. Difference spectroscopy is a convenient and adequate means of studying the formation of the said complexes.

[The content of pyridoxal coenzymes in the brain and liver of rats exposed to a single irradiation with x-rays].

A study was made of the effect of a single exposure of rats to 0.4 Gy X-radiation on the content of pyridoxal phosphate and pyridoxamine phosphate in gray and white brain substances and liver. At the same time changes were noted in the activity of pyridoxal kinase in the tissues under study.

[Detection of pulmonary tuberculosis in adolescents in institutions of the general medical network and efficacy of its treatment].

Altogether 100 adolescents with TB detected on referral to general medical institutions were followed up. 30 patients with a clear-cut TB clinicoroentgenological pictures were referred to special hospitals. The rest 70 adolescents were followed up in general outpatients departments.