Expression, purification and biophysical characterization of recombinant phospholipase PLA2 overproduced in .

The main purpose of this work was to develop new protocols for high yield purification of secretory phospholipase A2 (PLA2) and to investigate its biophysical properties. We have used a expression system for PLA2 expression and two-stage chromatography for its purification. The biophysical properties of PLA2 were investigated by circular dichroism.

The Continuum Approach to the Description of Semi-Crystalline Polymers Deformation Regimes: The Role of Dynamic and Translational Defects.

This paper presents a new approach to describe the mechanical behavior of semi-crystalline polymers, the plastic deformation of which is determined by their two-phase structure. To describe the plastic behavior of semi-crystalline polymers, a two-phase model is used. In the framework of this model, one phase is in a hard (crystalline) state, and the other in a soft (amorphous) state.