Influence of Biological Model on the Formation of the Pathogenic Properties of the West Nile Virus Isolate.

Various biological models are used to isolate West Nile virus, but their role as a selection factor that facilitates selection of isolates with certain properties is usually not evaluated. We compared pathogenic properties of three strains of the West Nile virus obtained from one sample of virus-containing material using different models: WNV Volgograd 900m/18 (on the model of suckling mice), WNV Volgograd 900a/18 (on C6/36 cells) and WNV Volgograd 900v/18 (on Vero cells). WNV Volgograd 900m/18 strain demonstrated virulent (LD 5×10±0.

Morphological features of doxorubicin-resistant Walker 256 carcinosarcoma and response of mast cells.

Background: The mechanisms of drug resistance of cancer have not been yet elucidated in details. Recently, the role of mast cells (MCs) in the development of drug resistance has been brought in the limelight. The aim of the study was to examine the morphological features of doxorubicin (DOX)-resistant Walker 256 carcinosarcoma and to assess the response of MCs and histamine content in these cells in relation to the development of resistance to DOX as well as in DOX-resistant tumors.

Analysis of malignancy-associated DNA changes in interphase nuclei of buccal epithelium in persons with breast diseases.

Aim: To study from the point of view of statistical and geometrical theory of pattern recognition, the peculiarities of the distribution of optical density of DNA in the interphase nuclei of mammary buccal epithelium upon pathology.

Methods: Cytogenetic investigation of buccal smears and computer-based image analysis were used.

Results: It is shown that in malignant neoplasms of the mammary glands compared with its values in benign, the optical density of DNA in the nuclei of buccal epithelium increase in a range from 0.

Analysis of malignancy-associated DNA changes in the nuclei of buccal epithelium in the pathology of the thyroid and mammary glands.

The object of the investigation reported in this paper was to study, from the point of view of statistical and geometric theory of pattern recognition, the DNA optical density distribution peculiarities in the interphase nuclei of buccal epithelium present in the pathology of the thyroid and mammary glands. Two new indices to characterize this distribution (ratio of modal class volumes and relief index) are proposed. It is shown that in malignant neoplasms of the thyroid and mammary glands the changes in the nuclei of buccal epithelium are characterized by an increase in the optical density of DNA over a range from 0.