Characteristics of heme oxygenase-1 expression in rat hepatocytes during the development of nonalcoholic steatohepatitis.

We studied heme oxygenase-1 expression in rat liver during the development of nonalcoholic steatohepatitis simulated by feeding high-fat ration for 30, 90, and 180 days. Increased number of hepatocytes synthesizing heme oxygenase-1 was demonstrated at all stages of pathology development. Herewith, the intensity of enzyme expression by individual cells was reduced on experimental day 180 during the development of liver fibrosis.

Simulation of nonalcoholic steatohepatitis in rats.

The new model of nonalcoholic steatohepatitis in rats is based on alimentary effects of hypercaloric diet including much cholesterol and beef fat. This model reproduces structural and functional disorders in the liver, including hepatocyte fatty degeneration, parenchymatous necrosis and fibrosis paralleled by dyslipidemia and is intended for studies of the mechanisms of formation, progress, and therapy of liver diseases of noninfectious origin.

[The regulatory role of the oxide nitrogen and monoxide carbon under oxidative stress in experimental dyslipidemia].

The condition of the system "prooxidants-antioxidants" in dynamic model alimentary dyslipidemia in Vistar rats of the lines were studied. General antioxidant activities, activities of antioxidant enzymes (katalase, glutathione reductase, glutathione peroxidase), levels of glutathione, products of lipoperoxides in blood and liver, intensive forming of the oxide nitrogen and monoxide carbon were investigated. The role of the signal molecules (oxide nitrogen and monoxide carbon) in regulation oxidative stress was discussed.