Imprinted proteins for determination of ovalbumin.

A strategy to design imprinted proteins (IPs) able to detect a glycoprotein (ovalbumin, OVA) was proposed. Glucose oxidase (GOx) was used as a matrix for obtaining the binding cavities with high specificity towards the template protein. The effective method to purify the obtained IPs from the template molecules was developed based on a combination of dialysis and gel filtration.

A novel electrochemical sensor for the detection of fipronil and its toxic metabolite fipronil sulfone using TiO-polytriazine imide submicrostructured composite as an efficient electrocatalyst.

For the first time, a simple and sensitive electrochemical sensor based on a screen printed electrode (SPE) modified with titanium dioxide (TiO) and polytriazine imide submicrostructured composite (TiO-PTI) has been developed for the simultaneous detection of fipronil (FIP) and its toxic metabolite fipronil sulfone (FIP-S). The submicrostructured composite material based on TiO and PTI was obtained by simple hydrothermal treatment of the Ti peroxocomplexes in the presence of pristine. This carbon nitride allotrope has better crystallinity and conductivity than its graphitic analog.

Structural and biochemical insights into CRISPR RNA processing by the Cas5c ribonuclease SMU1763 from Streptococcus mutans.

The cariogenic pathogen Streptococcus mutans contains two CRISPR systems (type I-C and type II-A) with the Cas5c protein (SmuCas5c) involved in processing of long CRISPR RNA transcripts (pre-crRNA) containing repeats and spacers to mature crRNA guides. In this study, we determined the crystal structure of SmuCas5c at a resolution of 1.72 Å, which revealed the presence of an N-terminal modified RNA recognition motif and a C-terminal twisted β-sheet domain with four bound sulphate molecules.

Recent advances in electrochemical monitoring of zearalenone in diverse matrices.

The current manuscript summarizes different electrochemical sensing systems developed within the last 5 years for the detection of zearalenone (ZEN) in diverse matrices such as food, feed, and biofluids. ZEN is one of the most prevalent non-steroidal mycotoxins that is often found in pre- and post-harvest crops. Crops contamination with ZEN and animal exposure to it via contaminated feed, is a global health and economic concern.

Homogenous FRET-based fluorescent immunoassay for deoxynivalenol detection by controlling the distance of donor-acceptor couple.

Semiconductor quantum dots (QDs) are one of the most popular luminescent labels that are widely used in food and medical analysis. Their unique optical properties establish QDs as excellent tools for highly sensitive biosensors based on Förster resonance energy transfer (FRET). To provide a convenient analytical system with long-term optical stability, a FRET pair consisting of QDs as energy donor and gold nanoparticles (GNs) as energy acceptor was developed.