Publications by authors named "N Uno"
Microcell-mediated chromosome transfer between non-identical human iPSCs.
Mol Ther Nucleic Acids
December 2024
Article Synopsis
- Microcell-mediated chromosome transfer (MMCT) is a new technique for manipulating chromosome numbers using human induced pluripotent stem cells (hiPSCs) to create disease models with hyperaneuploidy.
- The study highlights the efficiency of using hiPSCs as both donor and recipient cells in the chromosome transfer process, allowing for the tagging and transfer of specific chromosomes like Chr21 and ChrY using CRISPR-Cas9.
- This innovative method aims to improve the study of rare diseases and enhance our understanding of early development by integrating key chromosomal regions into hiPSCs.
Missing an entire chromosome or chromosome arm in normal diploid cells has a deleterious impact on cell viability, which may contribute to the development of specific birth defects. Nevertheless, the effects of chromosome loss in human cells have remained unexplored due to the lack of suitable model systems. Here, we developed an efficient, selection-free approach to generate partial monosomy in human induced pluripotent stem cells (iPSCs).
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- A new technology was developed to extend the lifespan and maintain the differentiation ability of human mesenchymal stem cells (hMSCs) using nonintegrative, temperature-sensitive Sendai virus vectors.
- This "rejuvenation" process allows for improved cell growth and cloning abilities while keeping the cells' normal characteristics and functionality.
- The method ensures that modified cells can be supplied for research and clinical applications without altering their genetic makeup, potentially benefiting drug development and cell therapy.
Article Synopsis
- - The research focuses on developing pan-coronavirus interventions by identifying 50 antibodies from human B cells, particularly highlighting the antibody 54043-5, which binds to a common part of spike proteins in various coronaviruses.
- - A structural analysis revealed that 54043-5 recognizes a specific, highly conserved region of the S2 subunit in SARS-CoV-2, which is critical for understanding how this antibody can potentially provide protection.
- - Although 54043-5 does not neutralize the virus directly, it activates immune responses that help combat infections, and certain modifications to this antibody showed protective effects in mouse models of SARS-CoV-2 disease.
Unlabelled: Development of next-generation influenza virus vaccines is crucial to improve protection against circulating and emerging viruses. Current vaccine formulations have to be updated annually due to mutations in seasonal strains and do not offer protection against strains with pandemic potential. Computationally optimized broadly reactive antigen (COBRA) methodology has been utilized by our group to generate broadly reactive immunogens for individual influenza subtypes, which elicit protective immune responses against a broad range of strains over numerous seasons.
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