In situ spatial transcriptomic analysis of human skeletal muscle using the Xenium platform.

Traditional transcriptomic studies often overlook the complex heterogeneity of skeletal muscle, as they typically isolate RNA from mixed muscle fibre and cell populations, resulting in an averaged transcriptomic profile that obscures fibre type-specific differences. This study assessed the potential of the recently developed Xenium platform for high-resolution spatial transcriptomic analysis of human skeletal muscle histological sections. Human vastus lateralis muscle samples from two individuals were analysed using the Xenium platform and Human Multi-Tissue and Cancer Panel targeting 377 genes complemented by staining of successive sections for Myosin Heavy Chain isoforms to differentiate between type 1 and type 2 muscle fibres.

Fascicle differentiation of upper extremity nerves on high-resolution ultrasound with multimodal microscopic verification.

This study aimed to compare the fascicular anatomy of upper limb nerves visualized using in situ high-resolution ultrasound (HRUS) with ex vivo imaging modalities, namely, magnetic resonance microscopy (MRM), histological cross-sections (HCS), and optical projection tomography (OPT). The median, ulnar, and superficial branch of radial nerve (n = 41) were visualized in 14 cadaveric upper limbs using 22-MHz HRUS. Subsequently, the nerves were excised, imaged with different microscopic techniques, and their morphometric properties were compared.

Attitudes of Slovenian students towards whole-body donation, organ donation, and the use of donated bodies in medical education.

Background: Anatomical dissection of whole bodies donated for teaching and research purposes remains a cornerstone of medical education. However, evolving sociocultural and ethical landscapes have historically influenced this practice, necessitating ongoing research to inform and enhance donation programs. This study examines the Slovenian students' attitudes and factors influencing their decision-making regarding body and organ donation for medical education.

Ultrasound Speckle Tracking Method Based on Gradient Optical Flow to Quantify Small Longitudinal Displacement, Shear and Longitudinal Strain in Peripheral Nerves.

Objective: This study aimed to develop, validate and test the clinical feasibility of ultrasound (US) speckle tracking method based on gradient optical flow for quantifying small longitudinal displacements, shear and strain in peripheral nerves.

Methods: The speckle tracking method was validated using seven thawed, fresh-frozen isolated cadaveric forearms. Longitudinal motion of the median nerve was captured using a high-frequency 22 MHz linear probe.

Fiber-type composition and 3D capillary analysis of the human splenius capitis muscle.

Despite the significance of neck muscles in musculoskeletal disorders, their microscopic anatomy remains poorly characterized. This study examined the splenius capitis muscle, focusing on its fiber-type composition, fiber size, and capillary network characteristics. For comparison and validation, the vastus lateralis muscle was also analyzed.